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Involvement of promyelocytic leukemia protein in the ethanol-induced apoptosis in mouse embryo fibroblasts

Cited 4 time in Web of Science Cited 4 time in Scopus
Authors
Wang, Li-Hui; Yang, Jing-Yu; Cui, Wei; Shin, Young Kee; Wu, Chun-Fu
Issue Date
2008-07
Publisher
PHARMACEUTICAL SOC JAPAN
Citation
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN; Vol.128, No.7, pp.1067-1071
Keywords
promyelocytic leukemia proteinapoptosisethanol
Abstract
The promyelocytic leukemia (PML) gene is a tumor suppressor gene associated with cell apoptosis, cell proliferation, and senescence. However, the role of PML in the ethanol-induced apoptosis is not fully-known. In this study, using wild-type mouse embryo fibroblasts (MEF) and PML null MEF cells, we found that (1) ethanol (100 mM and 200 mM) could obviously induce apoptosis of wild-type MEF cells, whereas, in PML null MEF cells, the pro-apoptotic function of ethanol was partially blocked; (2) the expression levels of phosphorylated p53 and two of its target genes, p21 and Bax, could be significantly up-regulated by ethanol (200 mM) in wild-type MEF cells in a time-dependent manner, but not in PML null MEF cells. These results indicate that PML plays an important role in ethanol-induced apoptosis, and p53-dependent apoptotic pathway may be involved in this process.
ISSN
0031-6903
Language
English
URI
http://hdl.handle.net/10371/80966
DOI
https://doi.org/10.1248/yakushi.128.1067
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College of Pharmacy (약학대학)Dept. of Pharmacy (약학과)Journal Papers (저널논문_약학과)
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