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Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads

Cited 43 time in Web of Science Cited 48 time in Scopus
Authors
Jun, Bong-Hyun; Kang, Homan; Lee, Yoon-Sik; Jeong, Dae Hong
Issue Date
2012-03
Publisher
MDPI
Citation
MOLECULES Vol.17 No.3, pp. 2474-2490
Keywords
복합학optically-encoded beadfluorescencequantum dotssurface-enhanced Raman
scattering (SERS)
bead-based assaylabel-free detectionhigh-throughput screening
Abstract
Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding.
ISSN
1420-3049
Language
English
URI
http://hdl.handle.net/10371/81874
DOI
https://doi.org/10.3390/molecules17032474
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College of Education (사범대학)Dept. of Science Education (과학교육과)Chemistry (화학전공)Journal Papers (저널논문_화학전공)
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