S-Space College of Education (사범대학) Dept. of Science Education (과학교육과) Chemistry (화학전공) Journal Papers (저널논문_화학전공)
Fluorescence-Based Multiplex Protein Detection Using Optically Encoded Microbeads
- Jun, Bong-Hyun; Kang, Homan; Lee, Yoon-Sik; Jeong, Dae Hong
- Issue Date
- MOLECULES Vol.17 No.3, pp. 2474-2490
- 복합학; optically-encoded bead; fluorescence; quantum dots; surface-enhanced Raman
scattering (SERS); bead-based assay; label-free detection; high-throughput screening
- Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based multiplex detection techniques. Among the various techniques for high-throughput protein screening, optically-encoded beads combined with fluorescence-based target monitoring have great advantages over the planar array-based multiplexing assays. This review discusses recent developments of analytical methods of screening protein molecules on microbead-based platforms. These include various strategies such as barcoded microbeads, molecular beacon-based techniques, and surface-enhanced Raman scattering-based techniques. Their applications for label-free protein detection are also addressed. Especially, the optically-encoded beads such as multilayer fluorescence beads and SERS-encoded beads are successful for generating a large number of coding.