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α-Tocopheryl Succinate Inhibits Osteoclast Formation by Suppressing Receptor Activator of Nuclear Factor-kappaB Ligand (RANKL) Expression and Bone Resorption

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Authors
Kim, Ha-Neui; Lee, Jong-Ho; Jin, Won Jong; Lee, Zang Hee
Issue Date
2012-11
Publisher
Korean Society for Bone and Mineral Research
Citation
JOURNAL OF BONE METABOLISM Vol.19 No.2, pp. 111-120
Keywords
의약학Alpha-tocopheryl succinateInterleukin-1OsteoclastOsteoporosisRANKL
Abstract
Objective: Osteoclasts are bone-resorbing multinucleated cells derived from the monocyte/macrophage lineage during
normal and pathological bone turnover. Recently, several studies revealed that alpha-tocopheryl succinate (αTP-suc)
have demonstrated potent anti-cancer activities in vitro and in vivo. However, the effects of αTP-suc on osteoclast
formation and bone resorption remain unknown. Thus, in this study, we examined the effects of αTP-suc on osteoclast
differentiation and bone resorbing activity in inflammatory bone loss model.
Methods: Osteoclast differentiation assay was performed by cocultures of mouse bone marrow cells and calvarial
osteoblasts in culture media including interleukin-1 (IL-1). Osteoclasts were stained for tartrate-resistant acid
phosphatase (TRAP). The level of receptor activator of nuclear factor-kappaB ligand (RANKL) mRNA was determined
by reverse transcriptase-polymerase chain reaction (RT-PCR). ICR mice were administered an intraperitoneal
injections of αTP-suc or dimethyl sulfoxide (DMSO) 1 day before the implantation of a freeze-dried collagen sponge
loaded with phosphate-buffered saline (PBS) or IL-1 over the calvariae and every other day for 7 days. The whole
calvariae were obtained and analyzed by micro-computed tomography (CT) scanning, and stained for TRAP.
Results: αTP-suc inhibits osteoclast formation in cocultures stimulated by IL-1 and decreased the level of expression
of RANKL mRNA in osteoblasts. In addition, administered intraperitoneal injections of αTP-suc prevented
IL-1-mediated osteoclast formation and bone loss in vivo.
Conclusion: Our findings suggest that αTP-suc may have therapeutic value for treating and preventing bone-resorptive
diseases, such as osteoporosis.
ISSN
1598-107X
Language
English
URI
http://hdl.handle.net/10371/82377
DOI
https://doi.org/10.11005/jbm.2012.19.2.111
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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