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Effects of GM-CSF gene transfer using silica-nanoparticles as a vehicle on white blood cell production in dogs

Cited 10 time in Web of Science Cited 12 time in Scopus
Authors
Choi, Eun Wha; Shin, Il Seob; Chae, Young Jin; Koo, Hye Cheong; Lee, Jong Hwa; Chung, Tae Ho; Park, Yong Ho; Kim, Dae-Yong; Hwang, Cheol Yong; Lee, Chang Woo; Youn, Hwa Young
Issue Date
2008-04-02
Publisher
Elsevier
Citation
Exp Hematol. 2008;36:807-815
Abstract
Objective. We sought to test two concepts: that nanoparticles can be used for in vivo gene
delivery and that canine granulocyte-macrophage colony-stimulating factor (GM-CSF)/nanoparticles
can have possibility to be used to treat transient (acute) canine leukopenia.
Materials and Methods. We have generated a novel fluorescent-silica nanoparticle binding of
canine GM-CSF gene; canine GM-CSF gene was inserted between the cytomegalovirus promoter
and poly-adenylation sequences of simian virus 40, and the gene construct was ligated
to fluorescent silica nanoparticles functionalized with tertiary amine.
Results. When the GM-CSF/nanoparticles were injected into normal dogs, the GM-CSF was
expressed in peripheral blood mononuclear cells for at least 9 days and there were significant
increases in white blood cell counts, as confirmed by complete blood count, differential count,
and flow cytometry. Significant increases in expression of major histocompatibility complex
class II on granulocytes and in serum GM-CSF were also observed. Readministration of
the nanoparticles was also effective and expression in various tissues was confirmed by reverse
transcriptase polymerase chain reaction.
Conclusions. These GM-CSF/nanoparticles may be useful for correction of acute leukopenia,
such as chemotherapy-induced myelosuppression without developing neutralizing antibodies.
ISSN
0301-472X
Language
English
URI
http://hdl.handle.net/10371/8280
DOI
https://doi.org/10.1016/j.exphem.2008.01.007
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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