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Characterization of a recombinant Newcastle disease virus vaccine strain
Cited 44 time in
Web of Science
Cited 49 time in Scopus
- Authors
- Issue Date
- 2008-09-03
- Publisher
- American Society for Microbiology
- Citation
- Clin. Vaccine Immunol. 15:1572-1579
- Abstract
- A recombinant La Sota strain (KBNP-C4152R2L) in which fusion (F) and hemagglutinin-neuraminidase
(HN) genes were replaced with those of a contemporary genotype VIId virus, KBNP-4152, has been developed.
To attenuate the virulence of the recombinant strain, the F cleavage motif was mutated from 112RRQKR116 to
112GRQAR116, and to reduce pathogenic instability, a codon which does not allow changes to basic amino acids
by single point mutation was inserted at codon 115. In addition a six-nucleotide sequence was inserted into the
intergenic region between matrix protein and F genes for attenuation without breaking the rule-of-six. The
HN protein length was increased from 571 to 577 as a marker. Serological tests revealed that the antigenicity
of KBNP-C4152R2L was similar to that of KBNP-4152 but distinct from that of the La Sota strain. KBNPC4152R2L
was avirulent (intracerebral pathogenicity index, 0.0; mean death time, >168 h) and stable in
pathogenicity through in vivo passages. The killed oil emulsion of and live KBNP-C4152R2L were completely
protective against mortality and egg drop caused by virulent strains, and KBNP-C4152R2L was applicable to
in ovo vaccination. Therefore, KBNP-C4152R2L is a promising vaccine strain and viral vector in terms of
antigenicity, productivity, safety, and pathogenic stability.
- ISSN
- 1556-6811
- Language
- English
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