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Biomolecular computation with molecular beacons for quantitative analysis of target nucleic acids

Cited 3 time in Web of Science Cited 2 time in Scopus
Authors
Lim, Hee-Woong; Lee, Seung Hwan; Yang, Kyung-Ae; Yoo, Suk-In; Park, Tai Hyun; Zhang, Byoung-Tak
Issue Date
2013-01
Publisher
Elsevier Ireland Ltd.
Citation
Biosystems Vol.111 No.1, pp. 11-17
Keywords
복합학Biomolecular computationMolecular beaconQuantitative analysisWeighted-sum
Abstract
Molecular beacons are efficient and useful tools for quantitative detection of specific target nucleic acids. Thanks to their simple protocol, molecular beacons have great potential as substrates for biomolecular computing. Here we present a molecular beacon-based biomolecular computing method for quantitative detection and analysis of target nucleic acids. Whereas the conventional quantitative assays using fluorescent dyes have been designed for single target detection or multiplexed detection, the proposed method enables us not only to detect multiple targets but also to compute their quantitative information by weighted-sum of the targets. The detection and computation are performed on a molecular level simultaneously, and the outputs are detected as fluorescence signals. Experimental results show the feasibility and effectiveness of our weighted detection and linear combination method using molecular beacons. Our method can serve as a primitive operation of molecular pattern analysis, and we demonstrate successful binary classifications of molecular patterns made of synthetic oligonucleotide DNA molecules. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
ISSN
0303-2647
Language
English
URI
https://hdl.handle.net/10371/83300
DOI
https://doi.org/10.1016/j.biosystems.2012.09.004
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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