Publications
Detailed Information
Acute liver toxicity by carbon tetrachloride in HSP70 knock out mice
Cited 22 time in
Web of Science
Cited 29 time in Scopus
- Authors
- Issue Date
- 2007-06-22
- Publisher
- Elsevier
- Citation
- Experimental and Toxicologic Pathology 2007;59:29-34
- Keywords
- Acute liver toxicity ; HSP70 ; Knock out ; Mice ; Carbon tetrachloride (CCl4)
- Abstract
- The effects of carbon tetrachloride (CCl(4)) treatment on acute liver damage in knock out (heat shock proteins -- HSP70-/-) mice and wild-type (C57BL/6) mice were examined. Acute liver injury was induced by a single intraperitoneal injection of 0.3 ML/kg CCl(4) in olive oil. Mice were sacrificed at 12, 24, 48 and 72 h after treatment. To assess hepatotoxicity, alanine transaminase, neutrophil infiltration and degree of necrosis were measured. Western blot analysis was employed for heat shock proteins. The result revealed that HSP70-/- mice showed higher alanine transaminase levels and a more severe degree of neutrophilic infiltration and necrosis than those of wild-type mice. Furthermore, HSP70-/- mice recovered more slowly from CCl(4) treatment. In HSP70-/- mice, HSP47 was overexpressed. Therefore, HSP70-/- mice could be an adequate model of acute liver toxicity study.
- ISSN
- 0940-2993
- Language
- English
- Files in This Item:
- There are no files associated with this item.
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.