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Anti-apoptotic mechanism of silkworm hemolymph in HeLa cell apoptosis

Cited 5 time in Web of Science Cited 7 time in Scopus
Authors
Rhee, Won Jong; Park, Ju Hyun; Hahn, Ji-Sook; Park, Tai Hyun
Issue Date
2013-09
Publisher
Elsevier Ltd.
Citation
Process Biochemistry Vol.48 No.9, pp. 1375-1380
Keywords
복합학Silkworm hemolymphAnti-apoptotic activityCaspaseCytochrome cBax translocation
Abstract
Silkworm hemolymph (SH) was found to exhibit anti-apoptotic activities in mammalian and insect cell systems. An anti-apoptotic mechanism of SH was investigated in a staurosporine-induced HeLa cell using flow cytometry, caspase assay, Immunoblot, and Immunochemistry. The addition of 5%SH to the medium resulted in lower intracellular activities of caspase-3 and caspase-9 after 0.6 mu M of staurosporine treatment; however, SH did not directly inhibit the activities of those enzymes. This suggests SH inhibits the event upstream of these caspase activation steps, such as mitochondrial level events. We found from Immunoblot and Immunochemistry that cytochrome c release from the mitochondria was blocked by SH. SH also inhibited Bax translocation to the mitochondria. On the contrary, SH did not block the apoptosis when Bax is not involved in promoting apoptosis. With these results, we propose that SH protects mitochondria from apoptosis signal via blocking Bax translocation, and the subsequent apoptotic events are then inhibited. The inhibition of apoptosis using SH and its components may lead to new approaches for the minimization of cell death during commercial animal cell cultures.
ISSN
1359-5113
Language
English
URI
https://hdl.handle.net/10371/90919
DOI
https://doi.org/10.1016/j.procbio.2013.06.018
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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