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Betaine protects against rotenone-induced neurotoxicity in PC12 cells

Cited 22 time in Web of Science Cited 26 time in Scopus
Authors
Im, A-Rang; Kim, Young-Hwa; Uddin, Md. Romij; Chae, Sungwook; Lee, Hye Won; Kim, Yun Hee; Kim, Yeong Shik; Lee, Mi-Young
Issue Date
2013-07-01
Publisher
Springer US
Citation
Cellular and Molecular Neurobiology Vol.33 No.5, pp. 625-635
Keywords
의약학ApoptosisBetaineMitochondrial dysfunctionNeuroprotectionRotenone
Abstract
Rotenone is an inhibitor of mitochondrial complex I-induced neurotoxicity in PC12 cells and has been widely studied to elucidate the pathogenesis of Parkinsons disease. We investigated the neuroprotective effects of betaine on rotenone-induced neurotoxicity in PC12 cells. Betaine inhibited rotenone-induced apoptosis in a dose-dependent manner, with cell viability increasing from 50 % with rotenone treatment alone to 71 % with rotenone plus 100-μM betaine treatment. Flow cytometric analysis demonstrated cell death in the rotenone-treated cells to be over 50 %; the number of live cells increased with betaine pretreatment. Betaine pretreatment of PC12 cells attenuated rotenone-mediated mitochondrial dysfunction, including nuclear fragmentation, ATP depletion, mitochondrial membrane depolarization, caspase-3/7 activation, and reactive oxygen species production. Western blots demonstrated activation of caspase-3 and caspase-9, and their increased expression levels in rotenone-treated cells; betaine decreased caspase-3 and caspase-9 expression levels and suppressed their activation. Together, these results suggest that betaine may serve as a neuroprotective agent in the treatment of neurodegenerative diseases.
ISSN
0272-4340 (print)
1573-6830 (online)
Language
English
URI
https://hdl.handle.net/10371/91115
DOI
https://doi.org/10.1007/s10571-013-9921-z
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College of Pharmacy (약학대학)Dept. of Manufacturing Pharmacy (제약학과)Journal Papers (저널논문_제약학과)
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