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Enzyme delivery using the 30Kc19 protein and human serum albumin nanoparticles
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Hong Jai | - |
dc.contributor.author | Park, Hee Ho | - |
dc.contributor.author | Kim, Jeong Ah | - |
dc.contributor.author | Park, Ju Hyun | - |
dc.contributor.author | Ryu, Jina | - |
dc.contributor.author | Choi, Jeongseon | - |
dc.contributor.author | Lee, Jongmin | - |
dc.contributor.author | Rhee, Won Jong | - |
dc.contributor.author | Park, Tai Hyun | - |
dc.creator | 박태현 | - |
dc.date.accessioned | 2014-04-02T00:54:52Z | - |
dc.date.available | 2014-04-02T00:54:52Z | - |
dc.date.created | 2018-07-09 | - |
dc.date.issued | 2014-02 | - |
dc.identifier.citation | BIOMATERIALS, Vol.35 No.5, pp.1696-1704 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://hdl.handle.net/10371/91297 | - |
dc.description.abstract | Nanoparticles have been widely used for delivering various chemical and biomolecular drugs, such as anti-cancer drugs and therapeutic proteins. Among nanoparticles, protein nanoparticles have advantages of non-cytotoxicity and biodegradability. In this study, a recombinant 30Kc19 protein was applied to human serum albumin (HSA) nanoparticles to enhance cellular uptake and stability of a nanoparticle cargo enzyme. The 30Kc19 protein, which originates from silkworm, has cell-penetrating and enzymestabilizing abilities. Therefore, 30Kc19-HSA nanoparticles were expected to enhance cellular uptake and stability of an enzyme loaded on the nanoparticles. Here, nanoparticles loaded with beta-galactosidase were prepared using the desolvation method. The 30Kc19-HSA nanoparticles were uniformly spherical in shape, dispersed evenly in phosphate buffered saline and cell culture media, and released beta-galactosidase in a sustained manner. The 30Kc19-HSA nanoparticles had negligible toxicity to animal cells and exhibited enhanced cellular uptake and intracellular stability of beta-galactosidase in HeLa and HEK293 cells when compared with those of HSA nanoparticles. These results suggest that 30Kc19-HSA protein nanoparticles could be used as a versatile tool for drug delivery to various cells. (C) 2013 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | en |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Enzyme delivery using the 30Kc19 protein and human serum albumin nanoparticles | - |
dc.type | Article | - |
dc.author.alternative | 이홍재 | - |
dc.author.alternative | 박희호 | - |
dc.author.alternative | 김정아 | - |
dc.author.alternative | 박주현 | - |
dc.author.alternative | 류지나 | - |
dc.author.alternative | 최정선 | - |
dc.author.alternative | 이종민 | - |
dc.author.alternative | 이원종 | - |
dc.author.alternative | 박태현 | - |
dc.identifier.doi | 10.1016/j.biomaterials.2013.11.001 | - |
dc.citation.journaltitle | BIOMATERIALS | - |
dc.identifier.wosid | 000330156100034 | - |
dc.identifier.scopusid | 2-s2.0-84890155567 | - |
dc.description.srnd | OAIID:oai:osos.snu.ac.kr:snu2014-01/102/0000002410/1 | - |
dc.description.srnd | SEQ:1 | - |
dc.description.srnd | PERF_CD:SNU2014-01 | - |
dc.description.srnd | EVAL_ITEM_CD:102 | - |
dc.description.srnd | USER_ID:0000002410 | - |
dc.description.srnd | ADJUST_YN:N | - |
dc.description.srnd | EMP_ID:A002014 | - |
dc.description.srnd | DEPT_CD:458 | - |
dc.description.srnd | CITE_RATE:7.604 | - |
dc.description.srnd | FILENAME:1. (2014.2) enzyme delivery using the 30kc19 protein and human serum albumin.pdf | - |
dc.description.srnd | DEPT_NM:화학생물공학부 | - |
dc.description.srnd | EMAIL:thpark@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | CONFIRM:Y | - |
dc.citation.endpage | 1704 | - |
dc.citation.number | 5 | - |
dc.citation.startpage | 1696 | - |
dc.citation.volume | 35 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Tai Hyun | - |
dc.identifier.srnd | 2014-01/102/0000002410/1 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | SILKWORM HEMOLYMPH | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | RECOMBINANT PROTEIN | - |
dc.subject.keywordPlus | HSA NANOPARTICLES | - |
dc.subject.keywordPlus | EPO PRODUCTION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
dc.subject.keywordAuthor | Protein | - |
dc.subject.keywordAuthor | Protein nanoparticle | - |
dc.subject.keywordAuthor | Drug delivery | - |
dc.subject.keywordAuthor | Drug release | - |
dc.subject.keywordAuthor | Enzyme stability | - |
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