S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Dept. of Chemical and Biological Engineering (화학생물공학부) Journal Papers (저널논문_화학생물공학부)
Microfluidic bead-based sensing platform for monitoring kinase activity
- Lee, Seung Hwan; Rhee, Hyun-Woo; van Noort, Danny; Lee, Hong Jai; Park, Hee Ho; Shin, Ik-Soo; Hong, Jong-In; Park, Tai Hyun
- Issue Date
- Biosensors and Bioelectronics, Vol.57 No.1, pp. 1-9
- Protein kinases control cellular functions by regulating protein phosphorylation. Monitoring protein kinase activity is essential for medical diagnosis and drug screening. Here, we present a novel microfluidic device for performing simple and versatile protein kinase assays, which utilizes a microbead-based chemosensor. An automatic mix-and-measure technique was achieved using integrated pneumatic valves. After mixing each reagent for the kinase assay, the mixture was transferred to the sensing chamber. Then, phosphorylated and fluorescence-labeled peptides were captured and detected by the chemosensor. A fluorescence signal was observed depending on the presence of the kinase. Furthermore, activities of various kinases in the cell lysate and the inhibitory effect of specific chemicals on the kinases were monitored. These results indicate that chemosensor-based microfluidic chips can be developed as a versatile kinase assay system. (C) 2014 Elsevier B.V. All rights reserved.
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