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Microfluidic bead-based sensing platform for monitoring kinase activity

Cited 14 time in Web of Science Cited 16 time in Scopus
Authors
Lee, Seung Hwan; Rhee, Hyun-Woo; van Noort, Danny; Lee, Hong Jai; Park, Hee Ho; Shin, Ik-Soo; Hong, Jong-In; Park, Tai Hyun
Issue Date
2014-07
Publisher
Elsevier
Citation
Biosensors and Bioelectronics, Vol.57 No.1, pp. 1-9
Keywords
복합학ProteinkinaseactivityKinase assayChemosensorMicrofluidic device
Abstract
Protein kinases control cellular functions by regulating protein phosphorylation. Monitoring protein kinase activity is essential for medical diagnosis and drug screening. Here, we present a novel microfluidic device for performing simple and versatile protein kinase assays, which utilizes a microbead-based chemosensor. An automatic mix-and-measure technique was achieved using integrated pneumatic valves. After mixing each reagent for the kinase assay, the mixture was transferred to the sensing chamber. Then, phosphorylated and fluorescence-labeled peptides were captured and detected by the chemosensor. A fluorescence signal was observed depending on the presence of the kinase. Furthermore, activities of various kinases in the cell lysate and the inhibitory effect of specific chemicals on the kinases were monitored. These results indicate that chemosensor-based microfluidic chips can be developed as a versatile kinase assay system. (C) 2014 Elsevier B.V. All rights reserved.
ISSN
0956-5663
Language
English
URI
https://hdl.handle.net/10371/92816
DOI
https://doi.org/10.1016/j.bios.2014.01.039
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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