S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 16 No.1 (1975)
토끼 소화관벽 임파조직의 용혈소 형성반응에 관한 면역학적 연구
Immunological and Histological Studies on the GALT and the Other Lymphoid Organs in the Rabbits
- 이종혁; 장가용; 이광호
- Issue Date
- 서울대학교 의과대학
- Seoul J Med, Vol.16 No.1, pp. 44-53
- As an experiment for the study of bursa equivaleot
organ in mammalia, the present experiment was
performed on the GALT of young adult rabbits
whether the cells of it respond to the antigenic
stimulation or not.
The experimental animals, weighed 1. 5 to 1.8kg,
were randomly divided into two groups. Experimental
group received a single injection of 1 ml of 20%
sheep erythrocytes intravenously. Control group
received a single injection of 1 ml of saline solut ion
intravenously. After the injection of the antigen
(or saline) the animals at various interval (from 1st
day to 21st day) and the palatine tonsil, Peyer's
patch, appendix, spleen , and thymus were removed.
The cell suspensions obtained from each organ was
used for the plaque technique and the paraffin
sections obtained from each organ were carried
out for the staining with methyl-green pyronin,
silver, and hematoxylin-eosin. Hemolysin-producing
cells were determined using a modification of Jerne
plaque technique and the results were expressed as
plaque-forming cells per 10' cells.
The results were as followings:
1. In the experimental group the spleen and
palatine tonsil produced a number of plaqueforming
cells. It reached to the maximum at 3
days after the injection of the antigen and
then decreased gradually.
2. By contrast, thymus, appendix, and PeyerS
patch did not produce the plaque•forming cells
as did the spleen and palatine tonsil.
3. The number of large pyroninophilic cells were
markedly increased in the extrafollicular area
of palatine tonsil and the periarteriallymphocyte
sheath of spleen after the antigenic stimulation.
It reached to the maximum at 3 days after the
injection of antigen and then decreased
4. Number of large pyroninophilic cells did not
change in the appendix, Peyers patch, and
thymus after the antigenic stimulation.
5. The diffuse lymphatic tissue of the extrafolli
cular area of palatine tonsil were more well
developed than that of appendix and PeyerS
These results suggest that the palatine tonsil in
the rabbits as well as spleen responds to the
antigenic stirn ulation, while the appendix and
Peyers patch do not.