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Identification and characterization of a novel cell-penetrating peptide of 30Kc19 protein derived from Bombyx mori

Cited 10 time in Web of Science Cited 11 time in Scopus
Authors
Park, Hee Ho; Sohn, Youngsoo; Yeo, Ji Woo; Park, Ju Hyun; Lee, Hong Jai; Ryu, Jina; Rhee, Won Jong; Park, Tai Hyun
Issue Date
2014-09
Publisher
Elsevier
Citation
Process Biochemistry, Vol.49 No.9, pp. 1516-1526
Keywords
복합학30Kc19 proteinCell-penetrating peptide (CPP)30Kc1945–57 (Pep-c19)Protein deliveryIn vivo penetration
Abstract
Cell-penetrating peptides (CPPs) or protein transduction domains (PTDs) have attracted increasing attention due to their high potential to deliver various, otherwise impermeable, bioactive agents, such as drugs and proteins across cell membranes. A number of CPPs have been discovered since then. Recently, 30Kc19 protein has attracted attention because it was the first cell-penetrating protein that has been found in insect hemolymph. Here, we report a cell-penetrating peptide derived from 30Kc19 protein, VVNKLIRNNKMNC, which efficiently penetrates cells when supplemented to medium for mammalian cell culture. Moreover, like other CPPs, this "Pep-c19" also efficiently delivered cell-impermeable cargo proteins, such as green fluorescent protein (GFP) into cells. In addition to the in vitro system, Pep-c19 exhibited the cell-penetrating property in vivo. When Pep-c19 was intraperitoneally injected into mice, Pep-c19 successfully delivered cargo proteins into various organ tissues with higher efficiency than the 30Kc19 protein itself, and without toxicity. Our data demonstrates that Pep-c19 has a great potential as a cell-penetrating peptide that can be used as a therapeutic tool to efficiently deliver different cell-impermeable cargo molecules into the tissues of various organs. (C) 2014 Elsevier Ltd. All rights reserved.
ISSN
0032-9592
Language
English
URI
http://hdl.handle.net/10371/94316
DOI
https://doi.org/10.1016/j.procbio.2014.05.008
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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