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Enhancement of human erythropoietin production in Chinese hamster ovary cells through supplementation of 30Kc19-30Kc6 fusion protein
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Hee Ho | - |
dc.contributor.author | Choi, Jeongseon | - |
dc.contributor.author | Lee, Hong Jai | - |
dc.contributor.author | Ryu, Jina | - |
dc.contributor.author | Park, Ju Hyun | - |
dc.contributor.author | Rhee, Won Jong | - |
dc.contributor.author | Park, Tai Hyun | - |
dc.date.accessioned | 2016-01-28T05:26:25Z | - |
dc.date.available | 2016-01-28T05:26:25Z | - |
dc.date.created | 2018-07-09 | - |
dc.date.issued | 2015-06 | - |
dc.identifier.citation | Process Biochemistry, Vol.50 No.6, pp.973-980 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | https://hdl.handle.net/10371/95455 | - |
dc.description.abstract | The importance of anti-apoptosis in mammalian cell culture has been widely recognized. We reported previously that expression of Bombyx mori 30 K genes in Chinese hamster ovary (CHO) cells increases recombinant protein production by inhibiting apoptosis and enhancing specific productivity. However, previous studies have shown expression of the anti-apoptotic 30Kc6 protein as inclusion bodies in Escherichia coil. 30Kc19 protein, another silkworm hemolymph protein, has cell-penetrating and recombinant protein productivity-improving properties, and we found that it improves soluble expression of its partner. In this study, we fused 30Kc6 with 30Kc19 as an expression partner to express a soluble fused protein and to deliver the protein to cells. Supplementing the recombinant 30Kc19-30Kc6 fusion protein in cell culture medium increased viability, effectively penetrated the cells, and inhibited CHO cell apoptosis by 68%. Moreover, the mitochondrial membrane potential and ATP generation also increased by 50% and 33%, respectively. Erythropoietin (EPO) productivity increased by >30% because of the anti-apoptotic effect and increased specific productivity. These results demonstrate the potential use of this fusion protein as a supplement in mammalian cell culture during production of biopharmaceutical proteins. (C) 2015 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Applied Science | - |
dc.title | Enhancement of human erythropoietin production in Chinese hamster ovary cells through supplementation of 30Kc19-30Kc6 fusion protein | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 박희호 | - |
dc.contributor.AlternativeAuthor | 최정선 | - |
dc.contributor.AlternativeAuthor | 이홍재 | - |
dc.contributor.AlternativeAuthor | 류지나 | - |
dc.contributor.AlternativeAuthor | 박주현 | - |
dc.contributor.AlternativeAuthor | 이원종 | - |
dc.contributor.AlternativeAuthor | 박태현 | - |
dc.identifier.doi | 10.1016/j.procbio.2015.03.008 | - |
dc.citation.journaltitle | Process Biochemistry | - |
dc.identifier.wosid | 000356120400013 | - |
dc.identifier.scopusid | 2-s2.0-84937760931 | - |
dc.description.srnd | OAIID:oai:osos.snu.ac.kr:snu2015-01/102/0000002410/7 | - |
dc.description.srnd | ADJUST_YN:N | - |
dc.description.srnd | EMP_ID:A002014 | - |
dc.description.srnd | DEPT_CD:458 | - |
dc.description.srnd | CITE_RATE:2.414 | - |
dc.description.srnd | DEPT_NM:화학생물공학부 | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | CONFIRM:Y | - |
dc.citation.endpage | 980 | - |
dc.citation.number | 6 | - |
dc.citation.startpage | 973 | - |
dc.citation.volume | 50 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Tai Hyun | - |
dc.identifier.srnd | 2015-01/102/0000002410/7 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | FREE SUSPENSION-CULTURE | - |
dc.subject.keywordPlus | SILKWORM HEMOLYMPH | - |
dc.subject.keywordPlus | BOMBYX-MORI | - |
dc.subject.keywordPlus | CHO-CELLS | - |
dc.subject.keywordPlus | 30KC6 GENE | - |
dc.subject.keywordPlus | RECOMBINANT PROTEIN | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | EPO PRODUCTION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordAuthor | 30Kc19-30Kc6 protein | - |
dc.subject.keywordAuthor | Soluble expression | - |
dc.subject.keywordAuthor | Anti-apoptosis | - |
dc.subject.keywordAuthor | Erythropoietin (EPO) | - |
dc.subject.keywordAuthor | Productivity | - |
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