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Effect of light intensity on the correlation between cell mass concentration and optical density in high density culture of a filamentous microorganism

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Authors
Yoon, Jong Hyun; Shin, Jong-Hwan; Park, Joon Ho; Park, Tai Hyun
Issue Date
2015
Publisher
Korean Institute of Chemical Engineers
Springer
Citation
Korean Journal of Chemical Engineering, vol.32 no.9, pp. 1842-1846
Keywords
High Cell DensityOptical Density (OD)Dry Cell Mass ConcentrationFilamentous MicroorganismCell Morphology
Abstract
We investigated the effect of light intensity on the correlation between optical density (OD) and cell mass concentration in high cell density culture of Anabaena variabilis. When the light intensity was gradually increased to maintain specific irradiation rate above 10 mmol/s/g dry cell, the dry cell mass concentration to OD ratio changed when the cell density became higher than OD 10, while the correlation was linear when light intensity remained constant after 2.5 days. When the OD was below 10, the dry cell concentration to OD ratio in unicells was the same as that of filaments, indicating that filament length, unicell size, and unicell dry cell concentration did not affect the dry cell concentration to OD ratio. Scanning electron microscope pictures revealed the differences in morphological structures between filaments below and above OD 10. Therefore, we propose that the morphological structures of filaments affected the dry cell concentration to OD ratio.
Language
English
URI
https://hdl.handle.net/10371/95456
DOI
https://doi.org/10.1007/s11814-015-0012-3
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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