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Genetic linkage mapping of RAPD segregating in Korean Ogol Chicken - White Leghorn backcross population

Cited 4 time in Web of Science Cited 7 time in Scopus
Authors
Hwang, K.C.; Song, K.D; Kim, T.H.; Jeong, D.K.; Sohn, S.H.; Lillehoj, H.S.; Han, Jae Yong
Issue Date
2001
Publisher
The Asian-australasian Association of Animal
Citation
Asian-australasian Journal of Animal Sciences, vol.14 no.3, pp. 302-306
Keywords
Genome MappingBackcross PopulationKOCRAPDMicrosatellitesLinkage Analysis
Abstract
This study was carried out to construct mapping population and to evaluate the methods involved, including polymorphic DNA marker system and appropriate statistical analysis. As an initial step to establish chicken genome mapping project, White Leghorn (WL) and Korean Ogol chicken (KOC) were used for generating backcross population. From 8 initial parents, total 280 backcross progenies were obtained and 40 were used for genotyping and linkage analysis. For development of novel polymorphic markers for KOC, Random Amplified Polymorphic DNA (RAPD) markers specific for this chicken line were generated. Also included in this study were six microsatellite markers from East Lansing map as reference loci. For segregation analysis, 15 RAPD markers and 6 microsatellites were used to genotype the backcross population. Among the RAPD markers that we developed, 2 pairs of markers were identified to be linked and another 4 RAPD markers showed linkage with microsatellites of known map. In summary, this study showed that our backcross population generated from the mating of KOC to WL serves as a valuable genetic resource for genotyping. Furthermore, RAPD markers are proved to be valuable in linkage mapping analysis.
ISSN
1011-2367
Language
English
URI
https://hdl.handle.net/10371/99037
DOI
https://doi.org/10.5713/ajas.2001.302
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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