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Characterization of recombinant scFv antibody reactive with an apical antigen of Eimeria acervulina

Cited 6 time in Web of Science Cited 7 time in Scopus
Authors
Min, Wongi; Kim, Jin-Kyoo; Lillehoj, Hyun Soon; Sohn, Eun Jung; Han, Jae Yong; Song, Ki Duk; Lillehoj, Erik Peter
Issue Date
2001
Publisher
Springer
Citation
Biotechnology Letters, vol.23 no.12, pp. 949-955
Keywords
apical antigenchickenEimeriarecombinant antibodyscFv
Abstract
The chicken monoclonal antibody (mAb), 6D-12-G10, reacts with an apical complex protein at the anterior tip of E. acervulina sporozoites that inhibits parasite invasion in vitro. Because this mAb was produced at low amount from the original hybridoma cells, an scFv antibody was constructed by amplification of the corresponding VH and VL genes and expressed in E. coli. The scFv antibody was produced at a minimum of 7 mg l−1 and exhibited virtually identical antigen reactivity as the original mAb.
ISSN
0141-5492
Language
English
URI
https://hdl.handle.net/10371/99039
DOI
https://doi.org/10.1023/A:1010531019633
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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