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Molecular Cloning and Characterization of cDNA Encoding Immunoglobulin Heavy and Light chain Variable Regions from Four Chicken Monoclonal Antibodies Specific to Surface Antigens of Intestinal Parasite, Eimeria acervulina

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Authors
Song, Ki Duk; Han, Jae Yong; Min, Wongi; Lillehoj, Hyun Soon; Kim, Sung Won; Kim, Jin-Kyoo
Issue Date
2001
Publisher
Springer
Citation
Journal of Microbiology, vol.39 no.1, pp. 49-55
Keywords
Eimeria acervulinachicken monoclonal antibodygene conversionantibody engineeringcomplementarity determining regionssomatic hypermutation
Abstract
We have developed four chicken hybridomas secreting monoclonal antibodies to induce a protective immune response against the chicken disease avian coccidiosis, caused by the intestinal parasite Eimeria
acervulina. However, since the amount of antibodies secreted from these hybridomas is too low or
sometimes they lost their ability to produce antibodies, the hybridoma method is not satisfactory in the
production of large amounts of chicken monoclonal antibodies. To bypass these problems, we applied
the antibody engineering technology using polymerase chain reaction. We cloned and determined the
sequences of variable domains of the four chicken monoclonal antibodies, namely, 2-1, 5D11, 13C8 and
8C3. The sequences comparison to germline sequences showed that the gene conversion mechanism
might contribute to developing diversification of heavy and λ-light chains in chicken antibodies. Several
pseudogene families regarded as donors in gene conversion were identified at each framework
region and the complementarity determining region of λ-light chains. In addition, as expected, numerous
changes of nucleotide sequences such as nucleotide substitution, insertion and deletion were found
predominantly in complementarity determining regions, which are likely to be somatic hypermutations
as a result of affinity maturation in antibody-producing cells.
ISSN
1225-8873
Language
English
URI
http://www.dbpia.co.kr/Journal/ArticleDetail/NODE01815168

https://hdl.handle.net/10371/99040
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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