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Detection of DNA fragment to differentiate Korean Cattle

Cited 5 time in Web of Science Cited 5 time in Scopus
Authors
Yeo, J.S.; Kim, J.W.; Chang, T.K.; Nam, D.H.; Han, Jae Yong; Choi, C.B.
Issue Date
2002
Publisher
The Asian-australasian Association of Animal
Citation
Asian-australasian Journal of Animal Sciences, vol.15 no.8, pp. 1071-1075
Keywords
RAPD-PCRSpecific DNA ProbeDNA FingerprintingHanwoo
Abstract
In order to identify and develop the specific DNA marker for the identification of Hanwoo (Korean Cattle) from other breeds, a specific DNA marker of 519 bp was identified and sequenced from polymorphic analysis using RAPD-PCR for 6 cattle breeds. Two different repetitive sequences, (AAC)5 and (GAAGA)2, were selected and designed to use specific probe to develop a DNA marker for Hanwoo specific. When the (AAC)5 probe was applied, the 10 kb specific DNA marker showed in the DNA fingerprinting from 237 of 281 Hanwoo individuals. This novel Hanwoo specific DNA probe is useful to perform the marker-assisted selection for screening Hanwoo purity as an unique genetic source.
ISSN
1011-2367
Language
English
URI
https://hdl.handle.net/10371/99041
DOI
https://doi.org/10.5713/ajas.2002.1071
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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