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Rapid sex identification of chicken by fluorescence in situ hybridization using a W chromosome-specific DNA probe

Cited 10 time in Web of Science Cited 9 time in Scopus
Authors
Sohn, S.H.; Lee, C.Y.; Ryu, E.K.; Han, Jae Yong; Multani, A.S.; Pathak, S.
Issue Date
2002
Publisher
The Asian-australasian Association of Animal
Citation
Asian-australasian Journal of Animal Sciences, vol.15 no.11, pp. 1531-1535
Keywords
ChickenW ChromosomeXhoI familyPCRFISHSexing
Abstract
It has been known that the sex of chicken cells can be most accurately identified by fluorescence in situ hybridization (FISH). However, the presently available FISH has not been widely used for sex identification, because the procedures for cell preparation and FISH itself are complicated and time-consuming. The present study was undertaken to test a rapid FISH procedure for sexing chicken. A FISH probe was simultaneously synthesized and labeled with digoxigenin by polymerase chain reaction (PCR) targeting a 416 bp segment of the 717 bp XhoI family fragment which is repeated over 10 thousand times exclusively in the W chromosome. Sexing by FISH was performed on cytological preparations of early embryos, adult lymphocytes and feather pulps of newly hatched chicks. The DNA probe hybridized to all types of uncultured interphase as well as metaphase female but not male cells that had been examined. Moreover, consistent with the known site of the XhoI family, the hybridization signal was localized to the pericentromeric region of the W chromosome. We, therefore, conclude that the present PCR-based FISH can be used as a rapid and reliable sex identification procedure for chicken.
ISSN
1011-2367
Language
English
URI
http://hdl.handle.net/10371/99043
DOI
https://doi.org/10.5713/ajas.2002.1531
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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