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Simple separation of chicken gonadal primordial germ cells with and without foreign genes
Cited 6 time in
Web of Science
Cited 7 time in Scopus
- Authors
- Issue Date
- 2002
- Publisher
- Wiley
- Citation
- Cell Biology International, vol.26 no.7, pp. 647-651
- Keywords
- primordial germ cell ; Ficoll ; neo gene ; GFP
- Abstract
- Simplicity is the key element of an inexpensive technique described that is superior in performance
to previous methods. It can make it the rapid method of choice to obtain reasonable yields
of purified primordial germ cells (PGCs) for immediate production of germline chimeric chickens
with integrated foreign genes. After Ficoll centrifugation, the purity of PGCs from gonads was
80.9 0.08% (mechanical) compared with 86.1 0.19% (enzymatic). GFP gene and lacZtransduced
chicken gonadal primordial germ cells (gPGCs) examined 72 h after transduction
had a transfection efficiency of 61% and 64%, respectively. After 10 days of G418 selection,
approximately 90 and 92% of pure gPGCs did not contain other cells following this Ficoll gradient centrifugation method of preparation.
- ISSN
- 1065-6995
- Language
- English
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