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Simple separation of chicken gonadal primordial germ cells with and without foreign genes

Cited 5 time in Web of Science Cited 6 time in Scopus
Authors
Jeong, Dong Kee; Hong, Yeong Ho; Han, Jae Yong
Issue Date
2002
Publisher
Wiley
Citation
Cell Biology International, vol.26 no.7, pp. 647-651
Keywords
primordial germ cellFicollneo geneGFP
Abstract
Simplicity is the key element of an inexpensive technique described that is superior in performance
to previous methods. It can make it the rapid method of choice to obtain reasonable yields
of purified primordial germ cells (PGCs) for immediate production of germline chimeric chickens
with integrated foreign genes. After Ficoll centrifugation, the purity of PGCs from gonads was
80.9 0.08% (mechanical) compared with 86.1 0.19% (enzymatic). GFP gene and lacZtransduced
chicken gonadal primordial germ cells (gPGCs) examined 72 h after transduction
had a transfection efficiency of 61% and 64%, respectively. After 10 days of G418 selection,
approximately 90 and 92% of pure gPGCs did not contain other cells following this Ficoll gradient centrifugation method of preparation.
ISSN
1065-6995
Language
English
URI
https://hdl.handle.net/10371/99045
DOI
https://doi.org/10.1006/cbir.2002.0928
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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