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Expression and functional characterization of recombinant chicken interferon-gamma
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, K.D. | - |
dc.contributor.author | Lillehoj, H.S. | - |
dc.contributor.author | Choi, K.D. | - |
dc.contributor.author | Zarlenga, D. | - |
dc.contributor.author | Han, J.Y. | - |
dc.date.accessioned | 2017-01-10T01:42:19Z | - |
dc.date.available | 2017-01-10T01:42:19Z | - |
dc.date.issued | 1997 | - |
dc.identifier.citation | Veterinary Immunology and Immunopathology, vol.58 no.3-4, pp. 321-333 | ko_KR |
dc.identifier.issn | 0165-2427 | - |
dc.identifier.uri | https://hdl.handle.net/10371/99048 | - |
dc.description.abstract | A cDNA encoding chicken interferon-gamma (chIFN-γ) was cloned from a CD4+ T-cell hybridoma by reverse transcription-polymerase chain reaction (RT-PCR) and expressed in Escherichia coli, COS- and CEC-32 fibroblast cell lines. In general, recombinant chicken IFN-γ (rchIFN-γ) expressed in the COS- and CEC-32 cell lines showed high bioactivity in vitro. The kinetics of IFN-γ gene expression were examined in concanavalin A (Con A)-activated spleen lymphocytes by Northern blot and RT-PCR. IFN-γ mRNA was detected as early as 30 min after Con A activation, reached peak expression at 2 h and then decreased starting at 4 h post Con A activation. A rabbit serum made to a synthetic peptide of IFN-γ immunoprecipitated a 60 kDa E. coli maltose-binding fusion protein of recombinant IFN-γ (MBP-IFN) and a 26–27 kDa secreted protein from COS cells and Con A-activated spleen cells. IFN-γ inhibited vesicular stomatitis virus (VSV) mediated cytotoxicity of chicken embryonic fibroblast (CEF) cells and upregulated the expression of many macrophage cell surface antigens, including class I and class II major histocompatibility complex (MHC) proteins. These results show that chicken IFN-γ possesses anti-viral activity and immunoregulates macrophage activities. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Elsevier | ko_KR |
dc.subject | Chicken interferon | ko_KR |
dc.subject | Recombinant interferon-gamma | ko_KR |
dc.subject | Ia antigen induction | ko_KR |
dc.subject | Interferon gene expression | ko_KR |
dc.subject | Macrophage activation factor | ko_KR |
dc.title | Expression and functional characterization of recombinant chicken interferon-gamma | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 한재용 | - |
dc.identifier.doi | 10.1016/S0165-2427(97)00034-2 | - |
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