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Production of germline chimeric chickens by transfer of cultured primordial germ cells

Cited 72 time in Web of Science Cited 88 time in Scopus
Authors
Chang, Il-Kuk; Jeong, Dong Kee; Hong, Yeong Ho; Park, Tae Sub; Kim Moon, Yangha; Ohno, Tadao; Han, Jae Yong
Issue Date
1997
Publisher
Wiley
Citation
Cell Biology International, vol.21 no.8, pp. 495-499
Keywords
primordial germ cellsgermline chimeratransgenic chicken
Abstract
Primordial germ cells (PGCs) from stage 27 (5.5-day-old) Korean native ogol chicken embryonic germinal ridges were cultured in vitro for 5 days. As in in vivo culture, these cultured PGCs were expected to have already passed beyond the migration stage. Approximately 200 of these PGCs were transferred into 2.5-day-old white leghorn embryonic blood stream, and then the recipient embryos were incubated until hatching. The rate of hatching was 58.8% in the manipulated eggs. Six out of 60 recipients were identified as germline chimeric chickens by their feather colour. The frequency of germline transmission of donor PGCs was 1.3–3.1% regardless of sex. The stage 27 PGCs will be very useful for collecting large numbers of PGCs, handling of exogenous DNA transfection during culture, and for the production of desired transgenic chickens.
ISSN
1065-6995
Language
English
URI
https://hdl.handle.net/10371/99049
DOI
https://doi.org/10.1006/cbir.1997.0173
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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