Browse

A recombinant human G-CSF/GM-CSF fusion protein from E-coli showing colony stimulating activity on human bone marrow cells

Cited 9 time in Web of Science Cited 8 time in Scopus
Authors
Lee, Ah Young; Chung, Hye Kyung; Bae, Eun Kyong; Hwang, Jung Suk; Sung, Bong Whan; Cho, Chung Won; Kim, Jin-Kyoo; Lee, Kyung; Han, Jae Yong; Lee, Choon-Taek; Youn, Hyun Joo
Issue Date
2003
Publisher
Springer
Citation
Biotechnology Letters, vol.25 no.3, pp. 205-211
Abstract
Granulocyte-Macrophage colony stimulating factor (GM-CSF) and Granulocyte colony stimulating factor (G-CSF) are cytokines involved in the differentiation of bone marrow progenitor cells into myeloid cells. They also activate mature myeloid cells to mediate a variety of antimicrobial activities and inflammatory responses. Recombinant GM-CSF and G-CSF proteins have been used to treat various diseases including cancer and hematopoietic diseases and to isolate peripheral blood progenitor cells for bone marrow transplantation. A plasmid construct expressing recombinant human G-CSF/GM-CSF fusion protein has now been prepared by linking the human G-CSF and GMCSF coding regions and the recombinant fusion protein has been successfully expressed in E. coli. The recombinant human G-CSF/GM-CSF fusion protein was extracted and purified from the cellular inclusion and refolded into the biologically active form to show colony stimulating activity. The recombinant fusion protein exhibited colony stimulating activity on human bone marrow cell cultures, indicating that the linkage of GM-CSF and G-CSF by a linker peptide may not interrupt activities of the cytokines in the fusion protein. The colony forming unit of the fusion protein was also higher than those of the cultures treated with the same molar numbers of the recombinant human GM-CSF and G-CSF separately, which suggests that the fusion protein presumably retains both G-CSF and GM-CSF activities.
ISSN
0141-5492
Language
English
URI
https://hdl.handle.net/10371/100194
DOI
https://doi.org/10.1023/A:1022346800375
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse