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Enriched gonadal migration of donor-derived gonadal primordial germ cells by immunomagnetic cell sorting in birds

Cited 38 time in Web of Science Cited 36 time in Scopus
Authors
Kim, Jin Nam; Kim, Mi A; Park, Tae Sub; Kim, Duk Kyung; Park, Hyun Jeong; Ono, Tamao; Lim, Jeong Mook; Han, Jae Yong
Issue Date
2004
Publisher
Wiley
Citation
Molecular Reproduction and Development, vol.68 no.1, pp. 81-87
Keywords
gonadal primordial germ cell (gPGC)immunomagnetic treatmentchickenquailgonadal migration
Abstract
This study was conducted to evaluate whether immunomagnetic treatment could improve the retrieval and migration capacity of avian gonadal primordial germ cells (gPGCs) collected from gonads in 5.5-day-old chick and 5-day-old quail embryos, respectively. Collected gPGCs were loaded into a magnetic-activated cell sorter (MACS) after being conjugated with specific gPGC antibodies and either MACS-treated or non-treated cells in each species were subsequently transferred to the recipient embryos. MACS treatment significantly (P<0.05) increased the population ratio of gPGCs in gonadal cells retrieved (0.74 to 33.4% in the chicken and 2.68 to 45.1% in the quail). This was due to decreased number of nongPGCs in total cell population. MACS treatment further enhanced gonadal migration of gPGCs transferred in both species (10% vs. 80–85% in the chicken and 10–15% vs. 70–80% in the quail). Increase in the number of microinjected cells up to 600 cells/embryo did not eliminate such promoting effect. In conclusion, MACS treatment greatly increased the population ratio of avian gPGCs in gonadal cells, resulting improved gonadal migration in recipient embryos.
ISSN
1040-452X
Language
English
URI
https://hdl.handle.net/10371/100202
DOI
https://doi.org/10.1002/mrd.20051
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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