S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Journal Papers (저널논문_식품·동물생명공학부)
Enriched gonadal migration of donor-derived gonadal primordial germ cells by immunomagnetic cell sorting in birds
- Kim, Jin Nam; Kim, Mi A; Park, Tae Sub; Kim, Duk Kyung; Park, Hyun Jeong; Ono, Tamao; Lim, Jeong Mook; Han, Jae Yong
- Issue Date
- Molecular Reproduction and Development, vol.68 no.1, pp. 81-87
- gonadal primordial germ cell (gPGC); immunomagnetic treatment; chicken; quail; gonadal migration
- This study was conducted to evaluate whether immunomagnetic treatment could improve the retrieval and migration capacity of avian gonadal primordial germ cells (gPGCs) collected from gonads in 5.5-day-old chick and 5-day-old quail embryos, respectively. Collected gPGCs were loaded into a magnetic-activated cell sorter (MACS) after being conjugated with specific gPGC antibodies and either MACS-treated or non-treated cells in each species were subsequently transferred to the recipient embryos. MACS treatment significantly (P<0.05) increased the population ratio of gPGCs in gonadal cells retrieved (0.74 to 33.4% in the chicken and 2.68 to 45.1% in the quail). This was due to decreased number of nongPGCs in total cell population. MACS treatment further enhanced gonadal migration of gPGCs transferred in both species (10% vs. 80–85% in the chicken and 10–15% vs. 70–80% in the quail). Increase in the number of microinjected cells up to 600 cells/embryo did not eliminate such promoting effect. In conclusion, MACS treatment greatly increased the population ratio of avian gPGCs in gonadal cells, resulting improved gonadal migration in recipient embryos.
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