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Production of Biofunctional Recombinant Human Interleukin 1 Receptor Antagonist (rhIL1RN) from Transgenic Quail Egg White

Cited 27 time in Web of Science Cited 31 time in Scopus
Authors
Kwon, Se Chang; Choi, Jin Won; Jang, Hyun-Jun; Shin, Sang Su; Lee, Seul Ki; Park, Tae Sub; Choi, In Young; Lee, Gwan Sun; Song, Gwonhwa; Han, Jae Yong
Issue Date
2010
Publisher
Society for the Study of Reproduction
Citation
Biology of Reproduction, vol.82 no.6, pp. 1057-1064
Keywords
chicken ovalbumin promoterembryogene regulationlentiviral vectorrhIL1RNoviducttransgenic quail
Abstract
Oviduct-specific expression of heterologous recombinant proteins in transgenic birds is a promising technology for the large-scale production of therapeutic proteins in eggs. We describe the production of recombinant human interleukin 1 receptor antagonist (rhIL1RN) in the eggs of transgenic quails. To drive tissue-specific expression of rhIL1RN, a 1.35-kb fragment of the chicken ovalbumin promoter, which contains both the steroid-dependent regulatory element and the negative regulatory element, was used. A transgenic quail was generated by microinjection of a concentrated stock of lentivirus into stage X blastodermal cells. A single copy of the transgene was integrated into the seventh intron of the gene for conserved oligomeric golgi complex protein 5 (COG5) on chromosome 1. As expected, rhIL1RN expression was restricted to oviductal tissue, and the amount of protein deposited in the eggs of homozygous transgenic quails ranged from 88.7 to 233.8 ng/ml. Transgene expression was conserved from the G1 generation to the G4 generation, and there was no evidence of transgene silencing. In a bioassay using the EL4.NOB-1/CTLL-2 coculture system, no significant difference was observed between the egg-produced rhIL1RN and a commercially available rhIL1RN (anakinra).
ISSN
0006-3363
Language
English
URI
https://hdl.handle.net/10371/100287
DOI
https://doi.org/10.1095/biolreprod.109.081687
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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