Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen

Cited 15 time in Web of Science Cited 17 time in Scopus
Ha, Na-Young; Shin, Hyun Mu; Sharma, Prashant; Cho, Hyun Ah; Min, Chan-Ki; Kim, Hong-il; Yen, Nguyen Thi Hai; Kang, Jae-Seung; Kim, Ik-Sang; Choi, Myung-Sik; Kim, Young Keun; Cho, Nam-Hyuk
Issue Date
BioMed Central
Journal of Nanobiotechnology, 14(1):76
Zinc oxide nanoparticleZnO binding peptideScrub typhusVaccine
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Zinc oxide nanoparticle (ZNP) has been applied in various biomedical fields. Here, we investigated the usage of ZNP as an antigen carrier for vaccine development by combining a high affinity peptide to ZNP.

A novel zinc oxide-binding peptide (ZBP), FPYPGGDA, with high affinity to ZNP (K

 = 2.26 × 106 M−1) was isolated from a random peptide library and fused with a bacterial antigen, ScaA of Orientia tsutsugamushi, the causative agent of scrub typhus. The ZNP/ZBP-ScaA complex was efficiently phagocytosed by a dendritic cell line, DC2.4, in vitro and significantly enhanced anti-ScaA antibody responses in vivo compared to control groups. In addition, immunization with the ZNP/ZBP-ScaA complex promoted the generation of IFN-γ-secreting T cells in an antigen-dependent manner. Finally, we observed that ZNP/ZBP-ScaA immunization provided protective immunity against lethal challenge of O. tsutsugamushi, indicating that ZNP can be used as a potent adjuvant when complexed with ZBP-conjugated antigen.

ZNPs possess good adjuvant potential as a vaccine carrier when combined with an antigen having a high affinity to ZNP. When complexed with ZBP-ScaA antigen, ZNPs could induce strong antibody responses as well as protective immunity against lethal challenges of O. tsutsugamushi. Therefore, application of ZNPs combined with a specific soluble antigen could be a promising strategy as a novel vaccine carrier system.
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