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Detection of aquaporin-4 antibody using aquaporin-4 extracellular loop-based carbon nanotube biosensor for the diagnosis of neuromyelitis optica

Cited 13 time in Web of Science Cited 17 time in Scopus
Authors
Son, Manki; Kim, Daesan; Park, Kyung Seok; Park, Tai Hyun; Hong, Seunghun
Issue Date
2015-11-12
Publisher
Elsevier B.V.
Citation
Biosensors and Bioelectronics Vol.78, pp. 87-91
Keywords
Neuromyelitis opticaAquaporin-4Carbon nanotubeField-effect transistor
Abstract
Here we propose a carbon nanotube (CNT) field-effect transistor (FET) functionalized with aquaporin-4 (AQP4) extracellular loop peptides for the rapid detection of AQP4 antibody without pretreatment. Neuromyelitis optica (NMO) is a rare disease of the central nerve system that affects the optic nerves and the spinal cord. NMO-IgG, a serum antibody in patients, is highly specific for NMO and targets AQP4. We synthesized AQP4 extracellular loop peptides, known as primary autoimmune target in NMO, and immobilized them onto CNT-FET. The sensor showed p-type FET characteristics after the functionalization of peptides. The sensor was able to detect antibody with a detection limit of 1 ng l-1 . Moreover, AQP4 antibody in human serum was detected without any pretreatment. These results indicate that the biosensor can be used for rapid and simple detection of NMO antibody.
ISSN
0956-5663
Language
English
URI
http://hdl.handle.net/10371/116990
DOI
https://doi.org/10.1016/j.bios.2015.11.029
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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