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Optimization of Microencapsulation of β-Lactoglobulin-Vitamin A : β-Lactoglobulin-Vitamin A의 미세캡슐화 조건 최적화
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 황금택 | - |
dc.contributor.author | 탕가문 | - |
dc.date.accessioned | 2017-07-19T11:57:23Z | - |
dc.date.available | 2017-07-19T11:57:23Z | - |
dc.date.issued | 2015-08 | - |
dc.identifier.other | 000000066684 | - |
dc.identifier.uri | https://hdl.handle.net/10371/133955 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 식품영양학과, 2015. 8. 황금택. | - |
dc.description.abstract | As one of essential nutrients, vitamin A is important for growth, development, immune and vision system. It is widely used in various types of foods as a nutritional supplement. However, the functional properties of vitamin A are not fully exhibited by its great reactivity and low stability. Microencapsulation of vitamin A could increase the stability of vitamin A, prevent light-induced degradation and oxidation, and disperse vitamin A in water-soluble compounds. β-Lactoglobulin (β-Lg) is the major whey protein in cow's milk and has a central cavity able to bind hydrophobic ligands such as vitamin A. Furthermore, using ultra-high pressure (UHP) treatment, which changes the conformation of β-Lg, could increase the binding ability of β-Lg to vitamin A. In this study, microencapsulation condition for vitamin A using β-Lg as a wall material was optimized using response surface methodology (RSM). In order to achieve a higher microencapsulation efficiency (MEE), β-Lg was treated by UHP before encapsulating vitamin A. The UHP treatment condition of β-Lg was optimized using orthogonal array design (OAD). The microstructures of the microcapsules of β-Lg-vitamin A and microcapsules of UHP treated
β-Lg-vitamin A were observed by transmission electron microscopy (TEM) and Fourier transform infrared (FT-IR) spectroscopy. Optimal conditions for microencapsulation of β-Lg-vitamin A were 4.74:1 of the molar ratio, 1.43 h, pH 6.87, and 48.68℃ determined by RSM. The MEE under the optimized condition was calculated as 82.2% and the experimental value was 81.5%. Optimal conditions for UHP treatment of β-Lg were 300 MPa, 20 min, and 20℃ determined by OAD. The experimental MEE under the optimized condition was 94.8%. The optimized microcapsules of β-Lg-vitamin A and microcapsules of UHP treated β-Lg-vitamin A observed by TEM were sphere-shaped in a regular order. Vitamin A was observed by FT-IR to be inserted in the central cavity of β-Lg. These results indicate that the microencapsulation conditions forβ-Lg and vitamin A were optimized by RSM, the UHP treatment conditions forβ-Lg were optimized by OAD, and the microcapsules were successfully formed. | - |
dc.description.tableofcontents | ABSTRACT I
CONTENTS IV LIST OF TABLES VII LIST OF FIGURES IX INTRODUCTION 1 MATERIALS AND METHODS 4 1. Chemicals and reagents 4 2. Microencapsulation of β-Lg-vitamin A 4 3. Determination of microencapsulated vitamin A 5 4. Determination of MEE 6 5. Optimization of microencapsulation condition of β-Lg-vitamin A 6 5.1 Selection of independent variables on MEE for RSM 6 5.2 RSM design and statistical analysis 7 6. Optimization of microencapsulation of vitamin A using UHP treatedβ-Lg 11 6.1 UHP treatment 11 6.2 Selection of independent variables on MEE for OAD 11 6.3 OAD design and statistical analysis 12 7. TEM and FT-IR observations of microencapsulated β-Lg-vitamin A 15 RESULTS AND DISCUSSION 16 1. Optimization of microencapsulation of β-Lg-vitamin A 16 1.1 Effects of independent variables on MEE 16 1.2 Optimization of microencapsulation of β-Lg-vitamin A 18 2. Optimization of microencapsulation of vitamin A using UHP treated β-Lg 23 2.1 Effect of UHP treatment conditions against β-Lg on the MEE of β-Lg-vitamin A 23 2.2 Optimization of microencapsulation of vitamin A using UHP treated β-Lg 26 3. Microstructures observed by TEM and FT-IR spectroscopy 30 REFERENCES 34 KOREAN ABSTRACT 38 | - |
dc.format | application/pdf | - |
dc.format.extent | 948842 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | β-lactoglobulin | - |
dc.subject | vitamin A | - |
dc.subject | microencapsulation efficiency | - |
dc.subject | ultra-high pressure | - |
dc.subject | response surface methodology | - |
dc.subject | orthogonal array design | - |
dc.subject.ddc | 641 | - |
dc.title | Optimization of Microencapsulation of β-Lactoglobulin-Vitamin A | - |
dc.title.alternative | β-Lactoglobulin-Vitamin A의 미세캡슐화 조건 최적화 | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | VIII, 39 | - |
dc.contributor.affiliation | 생활과학대학 식품영양학과 | - |
dc.date.awarded | 2015-08 | - |
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