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Preparation of fucose-containing monosaccharides from Undaria pinnatifida sporophyll and synthesis of fucosylated oligossacharide using Bifidobacterium longum RD47 : Undaria pinnatifida sporophyll으로부터 fucose 함유물의 추출과 Bifidobacterium longum RD47을 이용한 푸코실 올리고당의 합성
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- Authors
- Advisor
- 지근억
- Major
- 생활과학대학 식품영양학과
- Issue Date
- 2015-08
- Publisher
- 서울대학교 대학원
- Keywords
- Bifidobacterium ; oligosaccharide ; sporophyll of U.pinnatifida ; L-fucose
- Description
- 학위논문 (석사)-- 서울대학교 대학원 : 식품영양학과, 2015. 8. 지근억.
- Abstract
- Human milk oligosaccharides (HMOs) are mostly fucosylated at their non-reducing termini. HMOs are known to function as a prebiotic for bifidobacteria. L-fucose is major component of HMOs but very costly. Fucoidan is a group of marine sulfated polysaccharide of the cell-wall matrix of brown algae, containing large proportions of L-fucose. In this research, L-fucose containing monosaccharides (FCMs) were obtained from fucoidan. For the experiment, fucoidan was extracted from U. pinnatifida sporophyll using 0.03 M HCl. Crude fucoidan was purified by ion-exchange chromatography and hydrolyzed to obtain FCMs. The analysis of monosaccharides composition was performed by Bio-LC. FCMs were applied to gel permeation column and used as a substrate to synthesize FO using the crude enzyme extracts from Bifidobacterium longum RD47. The synthesized FO was detected by thin layer chromatography. In the application aspect, the FO may provide a new constituent for cost-effective prebiotic food ingredient.
- Language
- English
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