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Enhanced osteogenic commitment of murine mesenchymal stem cells on graphene oxide substrate
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Cited 34 time in Scopus
- Authors
- Issue Date
- 2018-01-02
- Publisher
- BioMed Central
- Citation
- Biomaterials Research, 22(1):1
- Keywords
- Graphene ; Osteogenesis ; Mesenchymal stem cells ; Graphene oxide
- Abstract
- Background
Tissue engineering is an interdisciplinary field that attempts to restore or regenerate tissues and organs through biomimetic fabrication of scaffolds with specific functionality. In recent years, graphene oxide (GO) is considered as promising biomaterial due to its nontoxicity, high dispersity, and hydrophilic interaction, and these characteristics are key to stimulating the interactions between substrates and cells.
Method
In this study, GO substrates were fabricated via chemically immobilizing GO at 1.0mg/ml on glass slides. Furthermore, we examined the osteogenic responses of murine mesenchymal-like stem cells, C3H10T1/2 cells, on GO substrates.
Results
C3H10T1/2 cells on GO substrates resulted in increased cell surface area, enhanced cellular adhesions, and instigated osteogenic differentiation. Furthermore, priming of C3H10T1/2 cells with chondrocyte-conditioned medium (CM) could further induce a synergistic effect of osteogenesis on GO substrates.
Conclusions
All of these data suggest that GO substrate along with CM is suitable for upregulating osteogenic responses of mesenchymal stem cells.
- ISSN
- 2055-7124
- Language
- English
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