Single step isolation and activation of primary CD3 + T lymphocytes using alcohol-dispersed electrospun magnetic nanofibers

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Kim, Kwanghee; An, Hyo Jin; Jun, Seung-Hyun; Kim, Tae-Jin; Lim, Seon Ah; Park, Gayoung; Na, Hyon Bin; Park, Yong Il; Hyeon, Taeghwan; Yee, Cassian; Bluestone, Jeffrey A.; Kim, Jungbae; Lee, Kyung-Mi

Issue Date
American Chemical Society
Nano Letters, Vol.12 No.8, pp.4018-4024
Electrospun polymer nanofibers with entrapped magnetic nanoparticles (magnetic NP-NF) represent a novel scaffold substrate that can be functionalized for single-step isolation and activation of specific lymphocyte subsets. Using a surface-embedded T cell receptor ligand/trigger (anti-CD3 monoclonal antibody), we demonstrate, as proof of principle, the use of magnetic NP-NF to specifically isolate, enrich, and activate CD3(+) T cells from a heterogeneous cell mixture, leading to preferential expansion of CD8(+)CD3(+) T cells. The large surface area, adjustable antibody density, and embedded paramagnetic properties of the NP-NF permitted enhanced activation and expansion; its use represents a strategy that is amenable to an efficient selection process for adoptive cellular therapy as well as for the isolation of other cellular subsets for downstream translational applications.
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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