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Chitosan-graft-spermine as a gene carrier in vitro and in vivo

Cited 49 time in Web of Science Cited 50 time in Scopus
Authors
Jiang, Hu-Lin; Lim, Hwang-Tae; Kim, You-Kyoung; Arote, Rohidas; Shin, Ji-Young; Kwon, Jung-Taek; Kim, Ji-Eun; Kim, Ji-Hye; Kim, Duyeol; Chae, Chanhee; Nah, Jae-Woon; Choi, Yun-Jaie; Cho, Chong-Su; Cho, Myung-Haing
Issue Date
2011-01
Citation
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, Vol.77 No.1, pp.36-42
Keywords
Gene therapyNon-viral gene deliveryChitosanSpermineCytotoxicityAerosol delivery
Abstract
Chitosan has been proposed as a non-viral gene carrier because of its biodegradable and biocompatible cationic polymeric properties. However, the transfection efficiency of chitosan-DNA complexes is still too low for clinical trials. To improve transfection efficiency, we prepared a chitosan-graft-spermine (CHI-g-SPE) copolymer by an imine reaction between periodate-oxidized chitosan and spermine. The CHI-g-SPE copolymer was complexed with plasmid DNA in various copolymer-DNA weight ratios, and the complexes were characterized. The CHI-g-SPE copolymer showed good DNA binding ability and high protection of DNA from nuclease attack. The CHI-g-SPE/DNA complexes had well-formed spherical shapes and a nanoscale size with homogenous size distribution. The CHI-g-SPE copolymer had low cytotoxicity and CHI-g-SPE/DNA complexes showed transfection efficiency that was enhanced over that of chitosan-DNA. Furthermore, aerosol delivery of CHI-g-SPE/GFP complexes showed higher GFP expression compared with chitosan/GFP complexes, without toxicity. Our results indicate that the CHI-g-SPE copolymer has potential as a gene carrier. (C) 2010 Elsevier B.V. All rights reserved.
ISSN
0939-6411
URI
https://hdl.handle.net/10371/172372
DOI
https://doi.org/10.1016/j.ejpb.2010.09.014
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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