Browse

Chitosan-graft-polyethylenimine for Akt1 siRNA delivery to lung cancer cells

Cited 73 time in Web of Science Cited 85 time in Scopus
Authors
Jere, Dhananjay; Jiang, Hu-Lin; Kim, You-Kyoung; Arote, Rohidas; Choi, Yun-Jaie; Yun, Cheol-Heui; Cho, Myung-Haing; Cho, Chong-Su
Issue Date
2009-08
Citation
International Journal of Pharmaceutics, Vol.378 No.1-2, pp.194-200
Keywords
Polymeric carrierNon-viral vectorSiRNA deliveryAktChitosanPolyethylenimineLung cancer
Abstract
Efficient delivery of small interfering RNA (siRNA) remains a challenging task in RNA interference (RNAi) studies. In this study, we used chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer composed of chitosan and low molecular weight polyethylenimine (PEI) for the delivery of siRNA. The CHI-g-PEI carrier formed stable complexes with siRNA with compact spherical morphology. CHI-g-PEI delivered EGFP siRNA (siGFP) silenced EGFP expression nearly 2.5 folds higher than PEI25K at 50 pM siGFP concentration. Cell viability was found to be 2 folds high with CHI-g-PEI carrier than PEI25K. Also, our CHI-g-PEI carrier efficiently delivered Akt1 siRNA (siAkt) and thereby silenced onco-protein Akt1. Silencing of this crucial cell survival protein significantly reduced the lung cancer cell survival and proliferation. Additionally, Akt1 protein knock-down decreased A549 cell malignancy and metastasis. These findings suggest that the CHI-g-PEI carrier efficiently and safely delivered siRNA. Moreover, CHI-g-PEI mediated Akt1 siRNA delivery may immerge as a viable approach for lung cancer treatment. (C) 2009 Elsevier B.V. All rights reserved.
ISSN
0378-5173
URI
https://hdl.handle.net/10371/172403
DOI
https://doi.org/10.1016/j.ijpharm.2009.05.046
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse