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Extraction and purification of ustiloxin A from rice false smut balls by a combination of macroporous resin and high-speed countercurrent chromatography

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Authors
Wang, Gang; He, Dan; Zhao, Fengchun; Hu, Junqiang; Lee, Yin-Won; Shi, Jianrong; Xu, Jianhong
Issue Date
2020-12-08
Publisher
BMC
Citation
Food Production, Processing and Nutrition. 2020 Dec 08;2(1):29
Keywords
Ustiloxin ARice false smutPreparative purificationMacroporous resinHigh-speed counter-current chromatographyPhytotoxicity assay
Abstract
Rice false smut is an emerging plant disease worldwide. Ustiloxin A (UstA) is the major mycotoxin found in rice false smut balls, which are fungal colonies in rice florets. In this study, a new method consisting of macroporous resin column chromatography and high-speed countercurrent chromatography (HSCCC) was developed for UstA separation. UstA was extracted by a 3.81% HCOOH solution and adsorbed by XAD-4 resin. UstA was then eluted by a 40% methanol solution supplemented with 0.1% trifluoroacetic acid (TFA). Further purification was achieved by HSCCC using a two-phase solvent system consisting of n-butanol/TFA/H2O (1/0.05/1, v/v/v). Under the optimized conditions, 225 mg of UstA was obtained with a purity of 97.39% in a single run, with a final recovery of 65.2%. An inhibitory effect on seed germination of wheat and maize caused by UstA was observed in a preliminary phytotoxicity assay.
ISSN
2661-8974
Language
English
URI
https://hdl.handle.net/10371/173893
DOI
https://doi.org/10.1186/s43014-020-00043-9
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Agricultural Biotechnology (농생명공학부)Journal Papers (저널논문_농생명공학부)
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