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Novel enzymatic cross-linking-based hydrogel nanofilm caging system on pancreatic beta cell spheroid for long-term blood glucose regulation

Cited 32 time in Web of Science Cited 32 time in Scopus
Authors

Kim, Minji; Kim, Hyunbum; Lee, Young-sun; Lee, Sangjun; Kim, Seong-Eun; Lee, Uk-Jae; Jung, Sungwon; Park, Chung-Gyu; Hong, Jinkee; Doh, Junsang; Lee, Dong Yun; Kim, Byung-Gee; Hwang, Nathaniel S.

Issue Date
2021-06
Publisher
American Association for the Advancement of Science
Citation
Science advances, Vol.7 No.26, p. eabf7832
Abstract
Pancreatic beta cell therapy for type 1 diabetes is limited by low cell survival rate owing to physical stress and aggressive host immune response. In this study, we demonstrate a multilayer hydrogel nanofilm caging strategy capable of protecting cells from high shear stress and reducing immune response by interfering cell-cell interaction. Hydrogel nanofilm is fabricated by monophenol-modified glycol chitosan and hyaluronic acid that cross-link each other to form a nanothin hydrogel film on the cell surface via tyrosinase-mediated reactions. Furthermore, hydrogel nanofilm formation was conducted on mouse beta cell spheroids for the islet transplantation application. The cytoprotective effect against physical stress and the immune protective effect were evaluated. Last, caged mouse beta cell spheroids were transplanted into the type 1 diabetes mouse model and successfully regulated its blood glucose level. Overall, our enzymatic cross-linking-based hydrogel nanofilm caging method will provide a new platform for clinical applications of cell-based therapies.
ISSN
2375-2548
URI
https://hdl.handle.net/10371/202460
DOI
https://doi.org/10.1126/sciadv.abf7832
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  • College of Engineering
  • Department of Materials Science & Engineering
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