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Graphene Oxide-Supported Microwell Grids for Preparing Cryo-EM Samples with Controlled Ice Thickness

Cited 2 time in Web of Science Cited 2 time in Scopus
Authors

Kang, Min-Ho; Park, Junsun; Kang, Sungsu; Jeon, Sungho; Lee, Minyoung; Shim, Ji-Yeon; Lee, Jeeyoung; Jeon, Tae Jin; Ahn, Min Kyung; Lee, Sung Mi; Kwon, Ohkyung; Kim, Byung Hyo; Meyerson, Joel R.; Lee, Min Jae; Lim, Kwang-Il; Roh, Soung-Hun; Lee, Won Chul; Park, Jungwon

Issue Date
2021-10
Publisher
WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Citation
Advanced Materials, Vol.33 No.43, p. 2102991
Abstract
Cryogenic-electron microscopy (cryo-EM) is the preferred method to determine 3D structures of proteins and to study diverse material systems that intrinsically have radiation or air sensitivity. Current cryo-EM sample preparation methods provide limited control over the sample quality, which limits the efficiency and high throughput of 3D structure analysis. This is partly because it is difficult to control the thickness of the vitreous ice that embeds specimens, in the range of nanoscale, depending on the size and type of materials of interest. Thus, there is a need for fine regulation of the thickness of vitreous ice to deliver consistent high signal-to-noise ratios for low-contrast biological specimens. Herein, an advanced silicon-chip-based device is developed which has a regular array of micropatterned holes with a graphene oxide (GO) window on freestanding silicon nitride (SixNy). Accurately regulated depths of micropatterned holes enable precise control of vitreous ice thickness. Furthermore, GO window with affinity for biomolecules can facilitate concentration of the sample molecules at a higher level. Incorporation of micropatterned chips with a GO window enhances cryo-EM imaging for various nanoscale biological samples including human immunodeficiency viral particles, groEL tetradecamers, apoferritin octahedral, aldolase homotetramer complexes, and tau filaments, as well as inorganic materials.
ISSN
0935-9648
URI
https://hdl.handle.net/10371/202914
DOI
https://doi.org/10.1002/adma.202102991
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  • College of Natural Sciences
  • School of Biological Sciences
Research Area Cryogenic Electron Microscopy (Cryo-EM), Structural Biology, 분자생물학, 생물물리학, 생화학

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