Publications

Detailed Information

Apatite-Coated Collagen Scaffold for Bone Morphogenetic Protein-2 Delivery

Cited 42 time in Web of Science Cited 50 time in Scopus
Authors

Yang, Hee Seok; La, Wan-Geun; Bhang, Suk Ho; Lee, Tae-Jin; Lee, Minhyung; Kim, Byung-Soo

Issue Date
2011-09
Publisher
MARY ANN LIEBERT, INC
Citation
TISSUE ENGINEERING PART A, Vol.17 No.17-18, pp.2153-2164
Abstract
Bone morphogenetic proteins (BMPs) are the most potent osteoinductive growth factors. BMP-2 is clinically used for spine fusion and bone fracture healing. Commercially available BMP-2 uses a type I collagen scaffold as a carrier, but it only releases BMP-2 for a short period of time, which may release the bone formation efficacy. In the present study, we hypothesize that apatite coating of a collagen scaffold increases the release period as well as the osteogenic efficacy of BMP-2. Apatite coating was achieved by incubating collagen scaffolds in simulated body fluids (SBFs). Apatite coating on collagen scaffolds was confirmed by X-ray diffraction, electron spectroscopy for chemical analysis, attenuated total reflectance-Fourier transform infrared spectroscopy, and scanning electron microscopy. The rate and period of BMP-2 release from apatite-coated collagen scaffolds varied depending on the concentration of SBFs used. The 5x and 10x SBF apatite-coated collagen scaffolds released 91.8%+/- 11.5% and 82.2%+/- 13.1% of their loaded BMP-2 over 13 days in vitro, respectively, whereas noncoated collagen scaffold released 98.3%+/- 2.2% over the initial one day. BMP-2 released from apatite-coated collagen scaffold significantly increased the alkaline phosphatase activity of cultured osteoblasts, compared with BMP-2 released from noncoated collagen scaffold. Computed tomography and histomorphometry showed that BMP-2 delivery using apatite-coated collagen scaffolds resulted in 2.5-fold higher bone formation volume and 4.0-fold higher bone formation area than BMP-2 delivery using noncoated collagen scaffolds. This study shows that simple apatite coating of a collagen scaffold results in a BMP-2 carrier that renders long-term release of BMP-2 and dramatically enhances osteogenic efficacy.
ISSN
1937-3341
URI
https://hdl.handle.net/10371/204339
DOI
https://doi.org/10.1089/ten.tea.2010.0702
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Related Researcher

  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area biomaterials, nanomedicine, regenerative medicine

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share