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Spinner-flask culture induces redifferentiation of de-differentiated chondrocytes
Cited 34 time in
Web of Science
Cited 32 time in Scopus
- Authors
- Issue Date
- 2011-04
- Publisher
- SPRINGER
- Citation
- BIOTECHNOLOGY LETTERS, Vol.33 No.4, pp.829-836
- Abstract
- Implantation of chondrocytes isolated from patients and expanded in number in vitro is being used to treat patients with cartilage injuries. However, chondrocytes de-differentiate during culture with several passages, and cartilage regenerated by implantation of de-differentiated chondrocytes may be suboptimal. Here, we show that a spinner-flask culture system induces formation of chondrocyte aggregates and redifferentiate de-differentiated chondrocytes. Spinner-flask cultures induced the aggregate formation of chondrocytes with passage 1 or 4. Importantly, spinner-flask cultures induced redifferentiation of the de-differentiated chondrocytes, as type I collagen expression was significantly lower and type II collagen expression was significantly higher in spinner flask-cultured chondrocytes than in monolayer-cultured chondrocytes. This system is easily scalable and could be feasible for clinical setting.
- ISSN
- 0141-5492
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