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Protein Immobilization on Aminated Poly(glycidyl methacrylate) Nanofibers as Polymeric Carriers

Cited 39 time in Web of Science Cited 43 time in Scopus
Authors
Ko, Sungrok; Jang, Jyongsik
Issue Date
2007-04-20
Publisher
American Chemical Society
Citation
Biomacromolecules 2007, 8, 1400-1403
Abstract
Recently, protein carriers based on nanomaterials have been highlighted in diverse biological applications such
as protein extraction, separation, and delivery due to their facile gravimetric sedimentation in the aqueous phase
and abundant surface functionalities, which were used as anchoring sites for proteins. From this viewpoint, poly-
(glycidyl methacrylate) nanofibers (PGMA NFs) can be an excellent candidate for protein support because PGMA
NFs possess the activated epoxide functional groups on the surface. In addition, cured PGMA NFs (PGMA-NH2
NFs) reveal different surface functionalities such as primary amine groups. They can be linked with carboxylated
proteins. Ferritin and streptavidin were selected as models of the pristine and biolinker-mediated proteins in this
experiment and immobilized onto PGMA NFs and aminated PGMA-NH2 NFs. The successful conjugations of
ferritin and streptavidin were confirmed with transmission electron microscopy and fluorescein-isothiocyanatetagged
molecules. Protein immobilization using the pristine and the cured PGMA NFs could be considered as an
outstanding protocol for facile protein delivery.
ISSN
1525-7797
Language
English
URI
https://hdl.handle.net/10371/61371
DOI
https://doi.org/10.1021/bm070077g
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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