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Characterization of two cucumber green mottle mosaic virus strains isolated in Korea
한국에서 분리한 오이녹반모자이크바이러스 두 계통의 특성 규명

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Authors
김상민
Advisor
김국형
Issue Date
2003
Publisher
서울대학교 대학원
Keywords
오이녹반모자이크바이러스Cgmmv게놈Genome염기서열Rt-pcr열처리Heat treatment불활성역전사 중합효소연쇄반응
Description
Thesis (master`s)--서울대학교 대학원 :농생명공학부,2003.
Abstract
In Korea, CGMMV was first reported in 1989 and caused widespread
Blood flesh in watermelon field with considerable economic losses in 1995.
Since then, viral disease on cucurbit plants in many fields are being
reported occasionally. The complete nucleotide sequences of the genomic
RNAs of Cucumber green mottle mosaic virus Korean oriental melon strain
(CGMMV-KOM) and Korean watermelon strain (CGMMV-KW) have been
determined and small size RNAs in virion of two CGMMV isolates were
characterized as the subgenomic RNAs.
Each CGMMV strain consists of 6,424 nucleotides of plus-strand
RNA genome. The CGMMV genome consists of 60 and 176 nucleotides of
5' and 3' non-translated regions (NTRs), respectively, and contains four putative open reading frames (ORF1-4). ORFs 1 to 4 encode for proteins of
129, 186, 29, and 17.4-kDa, respectively. Even though two isolates of
CGMMV, CGMMV-KOM and CGMMV-KW have similarity over 98.3%, they
showed different symptom on the inoculum leaves of Chenopodium
amaranticolor; CGMMV-KOM expressed no symptom but CGMMV-KW
expressed chlorotic ringspot.
Language
English
URI
http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000058934

https://hdl.handle.net/10371/67606
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Theses (Master's Degree_식품·동물생명공학부)
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