S-Space College of Medicine/School of Medicine (의과대학/대학원) Microbiology (미생물학전공) Journal Papers (저널논문_미생물학전공)
Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65)
- Issue Date
- Society for General Microbiology
- Int J Syst Evol Microbiol. 55(Pt 4), 1649-1656
- Bacterial Proteins/*genetics ; Chaperonins/*genetics ; DNA, Bacterial/analysis ; DNA, Ribosomal/analysis ; Deoxyribonucleases, Type II Site-Specific/metabolism ; Humans ; Molecular Sequence Data ; Mycobacterium/*classification/genetics ; Mycobacterium tuberculosis/classification/genetics ; Phylogeny ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA ; Species Specificity ; Bacterial Typing Techniques
- The nucleotide sequences (604 bp) of partial heat-shock protein genes (hsp65) from 161 Mycobacterium strains containing 56 reference Mycobacterium species and 105 clinical isolates were determined and compared. hsp65 sequence analysis showed a higher degree of divergence between Mycobacterium species than did 16S rRNA gene analysis. Generally, the topology of the phylogenetic tree based on the hsp65 DNA sequences was similar to that of the 16S rRNA gene, thus revealing natural relationships among Mycobacterium species. When a direct sequencing protocol targeting 422 bp sequences was applied to 70 non-tuberculous mycobacterium (NTM) clinical isolates, all NTMs were clearly identified. In addition, an XhoI PCR restriction fragment length polymorphism analysis method for the differentiation of Mycobacterium tuberculosis complex from NTM strains was developed during this study. The results obtained suggest that 604 bp hsp65 sequences are useful for the phylogenetic analysis and species identification of mycobacteria.
- 1466-5026 (Print)