S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Theses (Master's Degree_식품·동물생명공학부)
Isolation and characterization of (RS)-2-(4-Chloro-2-methylphenoxy)propionic acid-degrading bacteria from soils
토양에서 제초제 MCPP 분해 세균의 분리 및 생리유전학적 특성에 대한 연구
- Issue Date
- 서울대학교 대학원
- 제초제; (RS)-2-(4-Chloro-2-methylphenoxy)propionic acid; MCPP; Herbicide; 플라스미드; Bacteria; 16S rDNA; Enantioselective degradation; REP-PCR; 분해양상
- Thesis (master`s)--서울대학교 대학원 :농생명공학부,2003.
- Fifteen numerically dominant (RS)-2-(4-Chloro-2-methylphenoxy)propionic acid ( MCPP,
mecoprop ) degrading bacteria were isolated from soils, and their genetic and phenotypic
characteristics were investigated. The mecoprop-degrading microorganisms were ubiquitous in golf
course soils, while no positive degradation was observed from agricultural soils. The isolate
were able to utilize the herbicide MCPP as a sole source of carbon and energy. Nine out of the
fifteen isolates were able to degrade both (R)-mecoprop and (S)-mecoprop, five strains ( MP1, 4,
9, 18, 24 ) could degrade only (S)-mecoprop and one strain exhibited enantioselective
degradation of (R)-mecoprop. The isolates also vigorously utilized other phenoxyacid herbidides,
including 2,4-D, 2,4-DP, and MCPA, as sole carbon sources. Analysis of 16S rDNA sequences
indicated that the all isolates were related to members of the genus, Sphingomonas. The REP-PCR
analysis of the isolates revealed that some strains isolated from different soils exhibited
identical REP-PCR patterns. All of the isolates were observed to have plasmid DNAs. Isolates,
MP4, MP17, and MP24 identified as the same strains by 16s rDNA sequence and REP-PCR analyses
exhibited different plasmid profile and degradation properties to each other.
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