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Isolation and characterization of (RS)-2-(4-Chloro-2-methylphenoxy)propionic acid-degrading bacteria from soils : 토양에서 제초제 MCPP 분해 세균의 분리 및 생리유전학적 특성에 대한 연구
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- Authors
- Advisor
- 가종억
- Issue Date
- 2003
- Publisher
- 서울대학교 대학원
- Keywords
- 제초제 ; (RS)-2-(4-Chloro-2-methylphenoxy)propionic acid ; MCPP ; Herbicide ; 플라스미드 ; Bacteria ; 16S rDNA ; Enantioselective degradation ; REP-PCR ; 분해양상
- Description
- Thesis (master`s)--서울대학교 대학원 :농생명공학부,2003.
- Abstract
- Fifteen numerically dominant (RS)-2-(4-Chloro-2-methylphenoxy)propionic acid ( MCPP,
mecoprop ) degrading bacteria were isolated from soils, and their genetic and phenotypic
characteristics were investigated. The mecoprop-degrading microorganisms were ubiquitous in golf
course soils, while no positive degradation was observed from agricultural soils. The isolate
were able to utilize the herbicide MCPP as a sole source of carbon and energy. Nine out of the
fifteen isolates were able to degrade both (R)-mecoprop and (S)-mecoprop, five strains ( MP1, 4,
9, 18, 24 ) could degrade only (S)-mecoprop and one strain exhibited enantioselective
degradation of (R)-mecoprop. The isolates also vigorously utilized other phenoxyacid herbidides,
including 2,4-D, 2,4-DP, and MCPA, as sole carbon sources. Analysis of 16S rDNA sequences
indicated that the all isolates were related to members of the genus, Sphingomonas. The REP-PCR
analysis of the isolates revealed that some strains isolated from different soils exhibited
identical REP-PCR patterns. All of the isolates were observed to have plasmid DNAs. Isolates,
MP4, MP17, and MP24 identified as the same strains by 16s rDNA sequence and REP-PCR analyses
exhibited different plasmid profile and degradation properties to each other.
- Language
- English
- URI
- http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000057721
https://hdl.handle.net/10371/68521
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