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Soluble pig lymphocyte activation gene-3 (LAG-3; CD223) inhibits human-to-pig xenogeneic mixed lymphocyte reaction

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Authors
Kim, Seon-Soo; Byun, Hyun-Jung; Kim, Sang-Hoon; Lee, Han-Hyoung; Kim, Sang Joon; Chun, Taehoon; Park, Chung-Gyu; Lee, Suk Jun
Issue Date
2010-02
Publisher
SPRINGER
Citation
BIOTECHNOLOGY LETTERS; Vol.32 2; 203-208
Keywords
Co-receptorLymphocyte activation gene-3Mixed lymphocyte reactionXenogeneic responseT cell activationPig
Abstract
Lymphocyte activation gene-3 (LAG-3; CD223) is a transmembrane protein that is structurally similar to CD4. Since LAG-3 has a much higher binding affinity to MHC class II than that of CD4, several approaches using soluble LAG-3 were used to modulate immune responses by activation or inhibition of MHC class II expressing antigen presenting cells. In this study, we constructed soluble pig LAG-3 containing a critical binding site (D1 and D2 region) to MHC class II molecules, combined with a constant region of an immunoglobulin (Ig) heavy chain. Flow cytometry analyses indicated that soluble pig LAG-3 binds to both pig and human MHC class II molecules. Moreover, soluble pig LAG-3 can inhibit human lymphocyte proliferation in the human-pig xenogeneic mixed lymphocyte reaction in a dose-dependent manner. These results indicate that soluble pig LAG-3 may be useful for controlling the xenogeneic T cell immune responses between the human and pig.
ISSN
0141-5492
Language
English
URI
https://hdl.handle.net/10371/76941
DOI
https://doi.org/10.1007/s10529-009-0144-9
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College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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