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The Role of Indoleamine 2,3-Dioxygenase in Retinal Pigment Epithelial Cell-mediated Immune Modulation

Cited 6 time in Web of Science Cited 6 time in Scopus
Authors

Park, Choul Yong; Yang, Seung-Ha; Chuck, Roy S.; Gehlbach, Peter L.; Park, Chung-Gyu

Issue Date
2010-01
Publisher
Informa Healthcare
Citation
OCULAR IMMUNOLOGY AND INFLAMMATION; Vol.18 1; 24-31
Keywords
co-stimulatory molecule3-dioxygenasemajor histocompatibility complexIndoleamine 2Immune modulationretinal pigment epithelium
Abstract
Purpose: To evaluate the role of indoleamine dioxygenase (IDO) in human retinal pigment epithelial cell (RPE)-mediated immune modulation. Methods: The immunosuppression of cultured human RPEs (ARPE-19 cells) was assayed using the mixed lymphocyte reaction (MLR) harvested from CBA/J and BALB/c mice. The expression of critical immune modulatory molecules, such as class II major histocompatibility complex (MHC) molecules, co-stimulatory molecules (B7-1 and B7-2), and indoleamine dioxygenase (IDO), was examined by reverse transcriptase polymerase chain reaction, flow cytometry, and immunofluorescence staining with and without IFN-gamma stimulation. Results: RPEs expressed IDO and class II MHC molecules under IFN-gamma stimulation. However, B7-1 and B7-2 were not expressed. RPE significantly suppressed MLR in the absence of IFN-gamma prestimulation. This phenomenon was enhanced by IFN-gamma stimulation. The addition of 1-methyl tryptophan l into the culture medium successfully reversed RPE immunosuppression. Conclusions: The expression of IDO may in part explain RPE-mediated immune suppression effects.
ISSN
0927-3948
Language
English
URI
https://hdl.handle.net/10371/76944
DOI
https://doi.org/10.3109/09273940903326203
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College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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