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Ultrasensitive and Selective Recognition of Peptide Hormone Using Close-Packed Arrays of hPTHR-Conjugated Polymer Nanoparticles

Cited 42 time in Web of Science Cited 46 time in Scopus
Authors
Kwon, Oh Seok; Ahn, Sae Ryun; Park, Seon Joo; Song, Hyun Seok; Lee, Sang Hun; Lee, Jun Seop; Hong, Jin-Yong; Lee, James S.; You, Sun Ah; Yoon, Hyeonseok; Park, Tai Hyun; Jang, Jyongsik
Issue Date
2012-06
Publisher
American Chemical Society
Citation
ACS Nano Vol.6 No.6, pp. 5549-5558
Keywords
복합학hormone receptorfield-effect transistornanoparticleshormone sensorconducting polymer
Abstract
Recognition of diverse hormones in the human body is a highly significant challenge because numerous diseases can be affected by hormonal imbalances. However, the methodologies reported to date for detecting hormones have exhibited limited performance. Therefore, development of innovative methods is still a major concern in hormone-sensing applications. In this study, we report an immobilization-based approach to facilitate formation of close-packed arrays of carboxylated polypyrrole nanoparticles (CPPyNPs) and their integration with human parathyroid hormone receptor (hPTHR), which is a B-class family of G-protein-coupled receptors (GPCRs). Our devices enabled use of an electrically controllable liquid-ion-gated field-effect transistor by using the surrounding phosphate-buffered saline solution (pH 7.4) as electrolyte solution. Field-induced signals from the peptide hormone sensors were observed and provided highly sensitive and selective recognition of target molecules at unprecedentedly low concentrations (ca. 48 fM). This hormone sensor also showed long-term stability and excellent selectivity in fetal bovine serum. Importantly, the hormone receptor attached on the surface of CPPyNPs enabled GPCR functional studies; synergistic effects corresponding to increased hPTH peptide length were monitored. These results demonstrate that close-packed CPPyNP arrays are a promising approach for high-performance biosensing devices.
ISSN
1936-0851
Language
English
URI
https://hdl.handle.net/10371/83141
DOI
https://doi.org/10.1021/nn301482x
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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