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Antiproliferation Properties of Grain Sorghum Dry Distiller's Grain Lipids in Caco-2 Cells

Cited 11 time in Web of Science Cited 13 time in Scopus
Authors
Zbasnik, Richard; Carr, Timothy; Weller, Curtis; Hwang, Keum Taek; Wang, Lijun; Cuppett, Susan; Schlegel, Vicki
Issue Date
2009-10
Publisher
American Chemical Society
Citation
Journal of Agricultural and Food Chemistry Vol.57 No.21, pp. 10435-10441
Keywords
복합학Grain sorghumantiproliferationethanoldry distiller’s grainCaco-2 cells
Abstract
Antiproliferative properties of lipids extracted from grain sorghum (GS) dry distillers grain (DDG) were analyzed to determine the feasibility of developing GS coproducts as a source for human health dietary ingredients. The lipid extract of GS-DDG was delivered to human colon carcinoma (Caco-2) cells by solubilizing 0−1000 μg/mL of GS-DDG lipids in 100 μg/mL increments with micelles. A significant reduction in cell viability (25−50%) resulted at treatment levels of 400−1000 μg/mL GS-DDG lipids (p < 0.05). Alternatively, total protein levels of cells treated with 400, 500, and 600 μg/mL of GS-DDG lipid were not significantly different from the control, indicating cell growth during the treatment period. Total cell counts for the control were not significantly different from the GS-DDG lipid treated cells, but dead cell counts increased by 10% for the latter sample with a concomitant increase of the intercellular protein lactate dehydrogenase leakage (30−40%) in the medium. Preliminary analysis by the fluorescence-activated cell method (FACs) demonstrated that nonviable cells were in either the early apoptotic, late apoptotic, or necrotic stage post-treatment with 400, 500, and 600 μg/mL GS-DDG lipids. Physiochemical characterization of the GS-DDG lipids used for the antiproliferation study showed the presence of vitamin E (predominantly γ-tocopherol), triacylglycerides (predominantly linoleic acid), policosanols, aldehydes, and sterols (predominantly campesterol and stigmasterol), each of which or as synergistic/additive group of constituents may be responsible for the antiproliferative effect.
ISSN
0021-8561
Language
English
URI
https://hdl.handle.net/10371/83693
DOI
https://doi.org/10.1021/jf902136p
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College of Human Ecology (생활과학대학)Dept. of Food and Nutrition (식품영양학과)Journal Papers (저널논문_식품영양학과)
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