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High-throughput generation of spheroids using magnetic nanoparticles for three-dimensional cell culture

Cited 42 time in Web of Science Cited 41 time in Scopus
Authors
Kim, Jeong Ah; Choi, Jong-Ho; Kim, Minsoo; Rhee, Won Jong; Son, Boram; Jung, Hyun-Kyo; Park, Tai Hyun
Issue Date
2013-11
Publisher
Elsevier Ltd.
Citation
Biomaterials Vol.34 No.34, pp. 8555-8563
Keywords
복합학3-Dimensional (3-D) cell cultureMagnetic fieldMagnetic nanoparticlesSpheroidTissue engineering
Abstract
Various attempts have been made to develop three-dimensional (3-D) cell culture methods because 3-D cells mimic the structures and functional properties of real tissue compared with those of monolayer cultures. Here, we report on a highly simple and efficient 3-D spheroid generation method based on a magnetic pin-array system to concentrate magnetic nanoparticle-incorporated cells in a focal direction. This system was comprised only of external magnets and magnetically induced iron pins to generate a concentrated magnetic field for attracting cells in a focused direction. 3-D spheroid generation was achieved simply by adding magnetic nanoparticle-incorporated cells into a well and covering the plate with a magnetic lid. Cell clustering occurred rapidly within 5 min and created more compact cells with time through the focused magnetic force. This system ensured not only reproducible and size-controlled generation of spheroids but also versatile types of spheroids such as random mixed, core-shell, and fused spheroids, providing a very useful tool for various biological applications.
ISSN
0142-9612
Language
English
URI
http://hdl.handle.net/10371/90917
DOI
https://doi.org/10.1016/j.biomaterials.2013.07.056
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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