Publications

Detailed Information

High-throughput generation of spheroids using magnetic nanoparticles for three-dimensional cell culture

Cited 74 time in Web of Science Cited 78 time in Scopus
Authors

Kim, Jeong Ah; Choi, Jong-Ho; Kim, Minsoo; Rhee, Won Jong; Son, Boram; Jung, Hyun-Kyo; Park, Tai Hyun

Issue Date
2013-11
Publisher
ELSEVIER SCI LTD
Citation
BIOMATERIALS, Vol.34 No.34, pp.8555-8563
Abstract
Various attempts have been made to develop three-dimensional (3-D) cell culture methods because 3-D cells mimic the structures and functional properties of real tissue compared with those of monolayer cultures. Here, we report on a highly simple and efficient 3-D spheroid generation method based on a magnetic pin-array system to concentrate magnetic nanoparticle-incorporated cells in a focal direction. This system was comprised only of external magnets and magnetically induced iron pins to generate a concentrated magnetic field for attracting cells in a focused direction. 3-D spheroid generation was achieved simply by adding magnetic nanoparticle-incorporated cells into a well and covering the plate with a magnetic lid. Cell clustering occurred rapidly within 5 min and created more compact cells with time through the focused magnetic force. This system ensured not only reproducible and size-controlled generation of spheroids but also versatile types of spheroids such as random mixed, core-shell, and fused spheroids, providing a very useful tool for various biological applications. (C) 2013 Elsevier Ltd. All rights reserved.
ISSN
0142-9612
Language
English
URI
https://hdl.handle.net/10371/90917
DOI
https://doi.org/10.1016/j.biomaterials.2013.07.056
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share