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Screening of target-specific olfactory receptor and development ofolfactory biosensor for the assessment of fungal contamination in grain

Cited 19 time in Web of Science Cited 23 time in Scopus
Authors
Ahn, Jung Ho; Lim, Jong Hyun; Park, Juhun; Oh, Eun Hae; Son, Manki; Hong, Seunghun; Park, Tai Hyun
Issue Date
2015
Publisher
Elsevier
Citation
Sensors and Actuators B, vol.210, pp. 9-16
Keywords
Bioelectronic noseNanovesicleCarbon nanotubeFungal contamination1-Octen-3-ol
Abstract
We herein report an integrated olfactory system to carbon nanotube platforms for biosensing appli-cations. In particular, this system can be used for the real-time monitoring of fungal contamination ingrain through detecting 1-octen-3-ol, which is specifically generated from contaminated grain. A spe-cific human olfactory receptor (OR) that recognizes 1-octen-3-ol was found using a cyclic adenosinemonophosphate (cAMP) response element (CRE)-reporter gene assay. Then, OR-containing nanovesi-cles were produced from human embryonic kidney (HEK)-293 cells. The nanovesicles, which generateolfactory signals using endogenous cellular components and over-expressed ORs, were integrated intosingle-walled carbon nanotubes field-effect transistors (SWNT-FETs). The nanovesicles and SWNT-FETsplay roles in perceiving specific odorants, and in amplifying cellular signals, respectively. Thus, thenanovesicle-integrated device was able to detect 1-octen-3-ol with excellent sensitivity and selectiv-ity, similar to the original olfactory system. This system can be effectively utilized for the real-timemeasurement of fungal contamination in grain.
Language
English
URI
https://hdl.handle.net/10371/95457
DOI
https://doi.org/10.1016/j.snb.2014.12.060
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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