S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Dept. of Chemical and Biological Engineering (화학생물공학부) Journal Papers (저널논문_화학생물공학부)
Screening of target-specific olfactory receptor and development ofolfactory biosensor for the assessment of fungal contamination in grain
- Ahn, Jung Ho; Lim, Jong Hyun; Park, Juhun; Oh, Eun Hae; Son, Manki; Hong, Seunghun; Park, Tai Hyun
- Issue Date
- Sensors and Actuators B, vol.210, pp. 9-16
- We herein report an integrated olfactory system to carbon nanotube platforms for biosensing appli-cations. In particular, this system can be used for the real-time monitoring of fungal contamination ingrain through detecting 1-octen-3-ol, which is specifically generated from contaminated grain. A spe-cific human olfactory receptor (OR) that recognizes 1-octen-3-ol was found using a cyclic adenosinemonophosphate (cAMP) response element (CRE)-reporter gene assay. Then, OR-containing nanovesi-cles were produced from human embryonic kidney (HEK)-293 cells. The nanovesicles, which generateolfactory signals using endogenous cellular components and over-expressed ORs, were integrated intosingle-walled carbon nanotubes field-effect transistors (SWNT-FETs). The nanovesicles and SWNT-FETsplay roles in perceiving specific odorants, and in amplifying cellular signals, respectively. Thus, thenanovesicle-integrated device was able to detect 1-octen-3-ol with excellent sensitivity and selectiv-ity, similar to the original olfactory system. This system can be effectively utilized for the real-timemeasurement of fungal contamination in grain.
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